Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA sequencing (scRNAseq) data from postnatal day (P) 5 Nes-Cfp/+ cerebella from Wild type (non-irradiated) and Irradiated (at P1) pups

ABSTRACT: Single-cell RNA sequencing (scRNAseq) data from postnatal day (P) 5 Nes-Cfp/+ cerebella from Wild type (non-irradiated) and Irradiated (at P1) pups

PROVIDER: PRJNA723809 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single-cell RNA sequencing (scRNAseq) data from postnatal day (P) 5 Nes-Cfp/+ cerebella from Wild type (non-irradiated) and Irradiated (at P1) pups

Project description:We performed large-scale single cell profiling of dissociated whole postnatal day (P) 5 cerebella from 4 male NestinCFP/+ pups that were either irradiated at P1, or their control littermates in two replicate experiments.

2021-12-08 | GSE173091 | GEO

Cerebellar granule cell replenishment postinjury by adaptive reprogramming of Nestin+ progenitors

Project description:We report the application of RNA-sequencing for high-throughput profiling of gene expression in Nestin expressing cells of P5 mouse cerebellum Non-Irradiated or Irradiated at P1. By using the Nes-CFP reporter mouse line, we isolated Nes-CFP positive cells of P5 cerebellum to compare the transcriptomes between Nes-CFP positive cells from Non-irradiated cerebellum and cerebellum irradiated at P1.

2017-08-01 | GSE100941 | GEO

Effect of cFP on gene expression in Vibiro cholerae

Project description:Vibrio cholerae is a Gram negative, motile, facultative anaerobic bacterium, and the causative agent of cholera, a severe diarrhoeal disease, which untreated can rapidly lead to dehydration, hypotensive shock, and death. Cholera is a significant human disease that is estimated to affect 3-5 million people each year. The mechanism by which V. cholerae regulates virulence gene expression in vivo is unknown, but a number of studies have suggested that low molecular weight signally molecules may be important in modulating gene expression. cFP is a low molecular weight cyclic dipeptide produced by multiple Vibrio species. Evidence previously generated in our laboratory showed that cFP inhibited the production of the virulence factors cholera toxin (CT) and the toxin coregulated pilus (TCP) in O1 El Tor V. cholerae strain N16961 during growth under virulence gene inducing conditions. cFP inhibition of CT and TCP production correlated with reduced transcription of several regulators that belong to the ToxR regulon. To identify additional cFP-responsive genes we performed microarray experiments with the O1 El Tor V. cholerae strain N16961. In these experiments N16961 was grown under virulence gene inducing conditions in the presence and absence of cFP before RNA was extracted and hybridized to microarrays. The results showed that cFP positively affected the expression of the LysR-family regulatory protein LeuO. This finding suggests the possibility that LeuO may be mediating cFP-dependent regulation of gene expression in response to environmental cFP. V. cholerae N16961 was grown under AKI growth condition in the presence or absence of 1 mM cFP for 2.5 or 3 hours when total RNA was extracted, differentially labelled and hybridized to microarrays. Four independent experiments were performed.

2013-08-13 | E-GEOD-38272 | biostudies-arrayexpress

Effect of cFP on gene expression in Vibiro cholerae

2013-08-13 | GSE38272 | GEO

The fission yeast Schizosaccharomyces pombe: H3K9me2, Erh1-GFP and CFP-Mmi1 ChIP-chip

Project description:ChIP-chip analyses of H3K9me2 (in WT, erh1â??, mmi1â?? and ccr4â??), Erh1-GFP (in WT and mmi1â??) and CFP-Mmi1 (in WT) H3K9me2: WT vs erh1â??, WT vs mmi1â?? and WT vs ccr4â??; Erh1-GFP: WT vs mmi1â??; CFP-Mmi1 in WT, Biological replicates: 2

2016-06-15 | E-GEOD-76111 | biostudies-arrayexpress

Global Transcriptional analysis of human spinal cord and neocortical neuroepithelial stem (NES) cells

Project description:We report the transcriptional profile of human spinal cord and neocortical neuroepithelial stem cells (SC-NES and NCX-NES cells) to identify potential engraftment markers in the lesioned spinal cord. We report that cells with a homologous neuroanatomical origin robustly integrate in the host tissue compared to cells with a different regional identity. Specifically, we describe that SC-NES cells display elective integration properties in the lesioned spinal cord compared to NCX-NES cells and that these properties are due to a combination of intrinsic and extrinsic factors. Upon transplantation, SC-NES cells differentiate into neurons and glial cells and extend lond-distance axons, whereas NCX-NES cells remain undifferentiated and display poor integration properties. SC-NES cell upregulate genes related to neurogenesis. In particular. we emphasize the upregulation of MTURN (maturin), ATCAY, PTN (pleiotropin), KAL1 (anosmin), SYT4 and SYT13.

2018-08-29 | GSE107514 | GEO

Transcriptome analysis of single cell-derived hiPSC-lt-NES cell clones

Project description:To identify cell-populations within human iPSC-derived long-term self-renewing neural epithelial stem cells (hiPSC-lt-NES cells) which retain capacities to generate undesired grafts, we established single cell-derived hiPSC-lt-NES cell clones and performed gene expression microarray analysis.

2023-06-04 | GSE166051 | GEO

Transcriptome analysis on OVA-GA(10) immunized GA-CFP mice

Project description:The goal of the study is to perform unbiased transcriptome analysis on spinal cord samples of immunized GA-CFP mice to configure the effect of OVA-GA(10) vaccination in a C9orf72 mouse model.

2019-10-04 | GSE138413 | GEO

The fission yeast Schizosaccharomyces pombe: H3K9me2, Erh1-GFP and CFP-Mmi1 ChIP-chip

Project description:ChIP-chip analyses of H3K9me2 (in WT, erh1∆, mmi1∆ and ccr4∆), Erh1-GFP (in WT and mmi1∆) and CFP-Mmi1 (in WT)

2016-06-15 | GSE76111 | GEO

Transcriptome analysis of hiPSC derived lt-NES cells

2023-06-04 | GSE225719 | GEO