Project description:Purpose: The goal of this study was to track fluctuations in gene expression across all defined life cycle stages of the cattle lungworm Dictyocaulus viviparus. Methods: Batches of worms from a Hannover, Germany strain were harvested in duplicate as eggs, L1, L2, L3, L4, hypobiotic L5, mixed-sex L5, male L5, female L5, mature males and mature females. RNA was isolated, reverse transcribed and sequenced on the Illumina platform. Finally, reads were mapped to the D. viviparus draft genome assembly and read counts associated with each feature were used to estimate gene expression levels and predict differential gene expression at various transitional points across the life cycle. Results: Principal component analyses indicated that major gene expression shifts occur upon hatching, infection of the bovid host, and sexual maturation.
Project description:A 50-mer oligonucleotide microarray was designed for large-scale gene expression analysis in Z. viviparus. To measure the expression of the probes and the corresponding assembled transcripts, the pool of mRNA used for the sequencing was hybridized.
Project description:A 50-mer oligonucleotide microarray was designed for large-scale gene expression analysis in Z. viviparus. To measure the expression of the probes and the corresponding assembled transcripts, the pool of mRNA used for the sequencing was hybridized. 1 Sample hybridized to microarray used to evaluate the transcript assembly described in Kristiansson et al 2009.
