Project description:This study compares gene expression in three strains (CPL2H1, CPL2H1 gtf1/gtf1 and CPL2H1 otf1/otf1) with the genetic background of Candida parapsilosis CLIB 214 (CBS 604). Total RNA was isolated using hot acid phenol extraction. A TruSeq stranded mRNA library was sequenced on an Illumina NovaSeq 6000 system.
Project description:Candida parapsilosis CLIB 214 (CBS 604) was grown on synthetic media containing hydroxyaromatic compounds as a sole carbon source. The cells cultivated in medium containing galactose were used as a control. Total RNA was isolated using hot acid phenol extraction. A TruSeq stranded mRNA library was sequenced on an Illumina NovaSeq 6000 system.
