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Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system [WES]

ABSTRACT: Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system [WES]

PROVIDER: PRJNA728047 | ENA |

REPOSITORIES: ENA

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Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system [WES]

Project description:We transplanted a single HSC into irradiated mice, creating a clonal hematopoietic system. This allowed us to study mitotically stable allelic imbalances at peripheral compartments (T cells, B cells).

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Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system

Project description:Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system

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Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system [RNA-seq]

Project description:Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system [RNA-seq]

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Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system [RNA-seq]

Project description:We transplanted a single HSC into irradiated mice, creating a clonal hematopoietic system. Using RNA-Seq on the cDNA of the peripheral compartments (T cells, B cells), we studied mitotically stable transcriptional states of the alleles of the entire transcriptome on the differentiating HSC.

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Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system

Project description:This SuperSeries is composed of the SubSeries listed below.

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TRACE-Seq Reveals Clonal Reconstitution Dynamics of Gene Targeted Human Hematopoietic Stem Cells

Project description:We present Tracking Recombination Alleles in Clonal Engraftment using sequencing (TRACE-Seq), a novel methodology that utilizes barcoded rAAV6 donor template libraries, carrying either in-frame silent mutations or semi-randomized nucleotide sequences outside the coding region, to track the in vivo lineage contribution of gene targeted HSPC clones. By targeting the HBB gene with a rAAV6 donor template library consisting of ~20,000 possible unique exon 1 in-frame silent mutations, we track the hematopoietic reconstitution of HBB targeted myeloid-only, lymphoid-only, and multi-lineage repopulating human HSPC clones in immunodeficient mice.

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Clonal heterogeneity in melanoma

Project description:Genetic heterogeneity is an important feature of solid tumors, however it is often assumed that most cancer cell lines are genetically homogeneous. A disparity in genetic complexity between cell lines and the disease they model could result in problems such as in vitro preclinical experiments overstating the effectiveness of putative therapeutics. We therefore derived clonal sublines by single cell cloning of in house derived early passage melanoma cell lines (LM-MEL-series), and compared their genomes to each other and the parental cell line using Illumina 610-Quad SNP arrays.

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AThi oHSC ATlo oHSC WES - Identifying clonal differences or differences in mutation burden between autophagy engaging and non-engaging hematopoietic stem cells during mouse aging.

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Project description:Listeria Persistence

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