Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell analysis of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin intestinal adenoma cells at the age of 11 wks

ABSTRACT: Single-cell analysis of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin intestinal adenoma cells at the age of 11 wks

PROVIDER: PRJNA728137 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single-cell analysis of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin intestinal adenoma cells at the age of 11 wks

Project description:Comparison of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin adenoma cells at the age of 11 wks. 2-3 male mice and 3-10 tumors per group were used in the experiment. Experiment was repeated twice (ApcMin2 and VApcMinL2 samples).

2021-12-10 | GSE174097 | GEO

Single cell analysis of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin Epcam- cells and CD45-Epcam- stromal cells from lamina propria at the age of 11 wks.

Project description:Comparison of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin Epcam- cells and CD45-Epcam- stromal cells from lamina propria at the age of 11 wks. Two male mice per group was used in the experiment.

2021-12-10 | GSE173565 | GEO

Single cell analysis of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin Epcam- cells and CD45-Epcam- stromal cells from lamina propria at the age of 11 wks.

Project description:Single cell analysis of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin Epcam- cells and CD45-Epcam- stromal cells from lamina propria at the age of 11 wks.

| PRJNA726167 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Elf4fl/fl-Villin-Cre Livers Transcriptomes

Project description:Conclusion: Elf4fl/fl-Villin-Cre mice are more sensitive to alcohol induced liver steatosis

2022-05-18 | GSE192923 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Elf4fl/fl-Villin-Cre distal colon Transcriptomes

Project description:Conclusion: Elf4fl/fl-Villin-Cre mice are more sensitive to Dextran sulfate (DSS) induced colon tumorigenesis

2021-01-07 | GSE164355 | GEO

Analyzing the effect of Lef1 deletion in mouse intestinal adenoma cells with single-cell RNA sequencing

Project description:Somatic mutations in APC or CTNNB1 genes lead to aberrant Wnt signaling and colorectal cancer (CRC) initiation and progression. Activation of Wnt pathway leads to the formation of beta-catenin-T-cell factor/Lymphoid enhancer binding factor 1 (Tcf/Lef1) complexes that activate transcription of oncogenic target genes. Lef1 is the only member of the Tcf gene family that is not expressed in the normal intestine, but is induced during intestinal tumorigenesis. Thus, we wanted to assess the role of Lef1 using genetic mouse models of intestinal adenomas and scRNA-seq technology. Tumorigenesis was initiated by inducing Apc mutation in Lgr5+ stem cells. Intestinal EpCAM+ epithelial cells of Lgr5-CreERT;Apc fl/fl (LApc) mouse and Lgr5-CreERT;Apc fl/fl; Lef1 fl/fl (LApcL) mouse were used to analyze the effects of Lef1 deletion in intestinal adenoma cells. We used WT mice as a control to distinguish adenoma cells.

2021-12-10 | GSE179483 | GEO

Analyzing the effect of Lef1 deletion in mouse intestinal organoids with single-cell RNA sequencing

Project description:We wanted to assess the role of Lef1 in ex vivo organoids using genetic mouse models of intestinal adenomas and scRNA-seq technology. Tumorigenesis was initiated by inducing Apc mutation in Lgr5+ stem cells. Intestinal cells of Lgr5-CreERT;Apc fl/fl (LApc) mouse and Lgr5-CreERT;Apc fl/fl; Lef1 fl/fl (LApcL) mouse were used to generate adenoma organoids. Organoids were cultured without growth factors for three passages and dissociated with Tryple express. We used WT mice as a control to distinguish adenoma cells. WT organoids were cultured with growth factors.

2021-12-10 | GSE179853 | GEO

RNA-seq of WT, ELF4-/- CACO2 and Elf4fl/fl-Villin-Cre Livers Transcriptomes

Project description:RNA-seq of WT, ELF4-/- CACO2 and Elf4fl/fl-Villin-Cre Livers Transcriptomes

| PRJNA793825 | ENA

Gene expression data from mouse colon. AOM+DSS induced colitis. INSR fl/fl mice or villin-CRE INSR-/- mice

Project description:Gene expression data from mouse colon. AOM+DSS induced colitis. INSR fl/fl mice or villin-CRE INSR-/- mice

| PRJNA449720 | ENA

LEF1 in Burkitt lymphoma

Project description:Recently global gene expression profiling of patients samples lead to a molecular definition of Burkitt Lymphoma (BL) with lymphocyte enhancer-binding factor 1 (LEF1) as a signature gene. Here we report the discovery of nucleic LEF1 in a very high proportion of BL cases (15/18) and LEF1 target genes. Germinal center B cells were devoid of detectable nuclear LEF1 expression as mantle cell lymphoma (0/5), marginal zone lymphoma (0/6), follicular lymphoma (0/12) or diffuse large B cell lymphoma (DLBCL) (1/31). Using whole genome gene expression profiling after transient knockdown of LEF1 in BL cell lines, new LEF1 target genes were identified. The joint expression of these genes in primary BL samples shows that LEF1 is not only expressed aberrantly in BL but also transcriptionally active. Our study identified aberrantly expressed LEF1 and its target genes suggesting an important functional role in BLs. 3 biological replicates scrb siRNA vs LEF1 si RNA , 4 siRNA are pooled

2013-06-01 | E-GEOD-42637 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data