Project description:Gene BbSwi6 playes an essential role in asexual differentiation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal development was analyzed by using high throughput sequencing (RNA-Seq).
Project description:Beauveria basiana is a well-known filamentous entomopathogenic fungus. Oxidation tolerance is an important determinant to fungal pathogenicity and biocontrol potential. B. bassiana transcriptional co-activator multiprotein bridging factor 1 (BbMBF1) contributes to fungal resistance to the oxidative stress. The interactome of BbMBF1 was revealed by the qualitative proteomic analysis integrated with the immuno-precipitation. This study is sought to unveil the comprehensive protein interaction of BbMBF1 and isolate the BbMBF1-mediated transcription factor under oxidative stress.
Project description:Gene BbGlc8 playes an essential role in conidation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal conidiation was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in deveopment of fungal conida.
Project description:Gene BbMbp1 playes an essential role in asexual differentiation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal development was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in asexual deveopment of B. bassiana.
Project description:Gene BbabaA and BbbrlA play an essential role in conidation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal conidiation was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in deveopment of fungal conida.
Project description:The presence of genetic groups of the entomopathogenic fungus Metarhizium anisopliae in soil is shaped by its adaptability to specific soil and habitat types, and by soil insect populations. Although the entomopathogenic life style of this fungus is well studied, its saprophytic life style has received little consideration. While a set of functionally related genes can be commonly expressed for the adaptability of this fungus to different environments (insect cuticle, insect blood and root exudates), a different subset of genes is also expected for each environment. In order to increase the knowledge of the potential use of M. anisopliae as a rhizosphere competent organism, in this study we evaluated the genetic expression of this fungus while growing on plant root exudates in laboratory conditions during a time course.
Project description:Beauveria bassiana is an important entomopathogenic fungus for biocontrol of insect pests. Its growth and development are closely related to its biocontrol potential. Previous studies revealed that the autophagy gene Atg1 (a kinase) regulates the funfal development and conidia formation. In this study, the phosphoproteome and proteome were compared between the wild-type and the knockout strain. The first dataset (IPX0002729001) is data for comparative proteome; the second dataset (IPX0002729002) was the data for comparative phosphoproteome. In this study, Q ExActive HF mass spectrometer was used for analysis.
