Project description:RNA seq result shows that WT-AG-haESCs and DKO-AG-haESCs samples are clustered together using hierarchical cluster both in the all expression genes and imprinting genes. This suggests that DKO of DMRs of H19 and Gtl2 do not change the overall gene expression patterns in AG-haESCs. We used round spermatids as control. Using RNA-seq, profile of all the expression genes and imprinting genes beteween different samples were analysed.

2015-06-29 | E-GEOD-60072 | biostudies-arrayexpress

Characterization of microRNA signature in activated CD4+ T, polyclonal Th17 (Th17 poly), OVA-specific Th17 (Th17 Ag-sp) cell skewed cultures

Project description:To determine specific miRNAs for Th17 cells, we performed comparative miRNA screening of activated CD4+ T, polyclonal Th17 (Th17 poly) and OVA-specific Th17 (Th17 Ag-sp) cell skewed cultures or Th17 Ag-sp sorted cells. The expression of miRNAs analyzed by microarray revealed 27 miRNAs exclusively expressed in culture or sorted Th17 Ag-sp cell subset.

2021-01-01 | GSE109992 | GEO

RNA-Seq of BEAS-2B cells treated with low doses of Ag nanoparticles for 6 weeks

Project description:Human bronchial lung cells (BEAS-2B) were treated for 6 weeks with low doses (1 µg/mL) of Ag nanoparticles (10 nm citrate coated). At the end of the exposure control and treated samples were submitted for RNA-Seq analysis (Hiseq2500). The overall goal of this experiment was to gain an in-depth understanding of the transcriptomic changes induced by low-dose, long-term exposure of human lung cells to Ag nanoparticles and to generate hypotheses related to their mechanisms of toxicity.

2018-04-30 | E-MTAB-6321 | biostudies-arrayexpress

DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:We generated RRBS data to analyse the DNA methylation profiling among WT-AG-haESCs, DKO-AG-haESCs and round spermatids, we found deletion of H19 and Gtl2 DMRs do not change the methylation patterns in AG-haESCs base on all detected CpG sites.

2015-06-29 | GSE60075 | GEO

The transcriptional outputs of AP2 and AG in floral development at the genomic scale

Project description:To determine whether AP2 and AG have antagonistic effects on transcription, The gene expression profiles of Ler, ag-11, ag-11 ap2-35 and ag-11 ap2-43 were examined using RNA-seq.

2016-09-01 | GSE81205 | GEO

Dam-specific RNA-seq

Project description:Dam-specific RNA-seq

| PRJEB25687 | ENA

DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

2015-06-29 | E-GEOD-60075 | biostudies-arrayexpress

Umbelopsis ramanniana AG strain:AG

Project description:Umbelopsis ramanniana AG strain:AG Genome sequencing

| PRJNA196032 | ENA