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Single cell RNA sequencing of mice skin cells in DMBA/TPA-induced tumorigenesis model

ABSTRACT: Single cell RNA sequencing of mice skin cells in DMBA/TPA-induced tumorigenesis model

PROVIDER: PRJNA731503 | ENA |

REPOSITORIES: ENA

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Single cell RNA sequencing of mice skin cells in DMBA/TPA-induced tumorigenesis model

Project description:WT or Cd226-/- mice were treated with DMBA and TPA. Three weeks later, cells were isolated from mice back skin, and divided CD45+ and CD45- cells by cell sorter. CD45+ and CD45- cells were mixed in 1:1 ratio, and we performed scRNA sequencing of skin cells.

2021-10-26 | GSE174776 | GEO

Expression data from sorted epithelial CD34+ expressing cells from DMBA/TPA induced skin tumors

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Transcriptomal analysis of mouse healthy skin sample and of DMBA/TPA-induced skin Tumor

Project description:In this analysis we plan to identify the genes that are differentially expressed during the development of skin carcinoma (SCC) subsquent to the chronic and topical application of a carcinogene agent (DMBA) and a tumor promoter (TPA)

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Integrin α3β1-dependent mRNA expression profile of hair bulge keratinocytes and their progeny during DMBA/TPA-mediated skin tumorigenesis

Project description:Integrin α3β1 in hair bulge population affects the tumor formation following DMBA/TPA carcinogenesis protocol, however linage tracing showed that hair bulge cells do not largely contributre to tumor mass. To investigate whether α3β1 might affect tumor promoting environment and to better understand its role in hair bulge population during skin tumorigenesis, we determined the expresison profile of hair bulge-originating keratinocytes isolted from α3β1KO and WT mice during initiation of tumorigenesis.

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Single-cell RNA (10X Chromium) of mouse DMBA/TPA treated or control back skin and matched livers (2) (IKMB) 2022

Project description:We generated single cell transcriptomes from full thickness skin biopsies in mouse to quantify the skin cell types found in this species (control samples). To study how mouse skin changes upon exposure to a carcinogen, we performed a classical two-stage skin carcinogenesis experiment, wherein cancer is initiated by a single application of 7,12-dimethylbenz[a]-anthracene (DMBA) followed by repeated treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) to drive cell proliferation. After 10 weeks, full thickness skin biopsies were collected and used to generate single cell transcriptomes (treatment samples).

2023-11-21 | E-MTAB-12456 | biostudies-arrayexpress

Single-cell RNA (10X Chromium) of mouse DMBA/TPA treated or control back skin and matched livers (1) (DKFZ) 2022

2023-11-21 | E-MTAB-12454 | biostudies-arrayexpress

Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (Essen) 2022

Project description:We generated single cell transcriptomes from full thickness skin biopsies in naked mole-rat to quantify the skin cell types found in this species (control samples). To study if and how naked mole-rat skin changes upon exposure to a carcinogen, we performed a classical two-stage skin carcinogenesis experiment traditionally performed in mice, wherein cancer is initiated by a single application of 7,12-dimethylbenz[a]-anthracene (DMBA) followed by repeated treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) to drive cell proliferation. After 12 weeks, full thickness skin biopsies were collected and used to generate single cell transcriptomes (treatment samples).

2023-11-21 | E-MTAB-12459 | biostudies-arrayexpress

Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (CRUK B) 2022

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Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (CRUK A) 2022

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Differentially expressed genes induced by TPA stimulation between the P2ry6+/+ and P2ry6-/- mice

Project description:We report that P2ry6-deficient mice exhibit marked resistance to 7,12-dimethylbenzanthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA)-induced two-stage skin papilloma formation compared with wild-type mice. Consistent with these findings, epidermal hyperplasia in response to TPA stimulation was suppressed in the P2ry6 knockout or MRS2578 (P2RY6 antagonist)-treated mice. The dramatic decrease in hyperplasia and tumorigenesis due to P2ry6 disruption was associated with the suppression of TPA-induced keratinocyte proliferation and inflammatory reactions.We have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish differentially expressed genes induced by TPA stimulation between the P2ry6+/+ and P2ry6-/- mice. Notably, P2ry6 deletion prevented the TPA-induced increase in YAP nuclear accumulation and hence its downstream gene expression in an MST/LATS1-dependent manner.

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