Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing (NGS) for quantitative transcriptomic analysis of Francisella strains (Francisella novicida, ribD-deficient Francisella novicida , Francisella tularensis)

ABSTRACT: Next Generation Sequencing (NGS) for quantitative transcriptomic analysis of Francisella strains (Francisella novicida, ribD-deficient Francisella novicida , Francisella tularensis)

PROVIDER: PRJNA731533 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Intra-macrophage Expression data of Francisella tularensis novicida U112

2017-05-02 | GSE75689 | GEO

Next Generation Sequencing (NGS) for quantitative transcriptomic analysis of Francisella strains (Francisella novicida, ribD-deficient Francisella novicida , Francisella tularensis)

Project description:To understand differences of gene expression profiles between Francisella strains RNA profiles of Francisella strains were generated by deep sequencing, in triplicate, using NovaSeq6000. qRT–PCR validation was performed using SYBR Green assays. Our study represents the first detailed differential transcriptomic analysis of Francisella strains , with biologic replicates, generated by RNA-seq technology.

2022-05-31 | GSE174793 | GEO

Intra-macrophage Expression data of Francisella tularensis novicida U112

Project description:These samples are part of an experiment comparing the expression profiles of Francisella tularensis novicida grown in chemically defined medium and bacteria isolated 24 hours post infection of J774 macrophages to identify virulence factors Custom microarray submitted previously was used as the platform (GPL20119). The samples submitted here were compared with samples submitted previously (GSM1673555-57 in GSE68478) and differentially expressed genes during the intra-macrophage growth were identified.

2017-05-02 | E-GEOD-75689 | biostudies-arrayexpress

Microarray analysis of human monocytes infected with Francisella tularensis

2008-08-13 | GSE12108 | GEO

Francisella tularensis subsp. novicida U112

Project description:Francisella tularensis subsp. novicida sRNA sequencing

| PRJNA314067 | ENA

Transcription profiling of human monocytes infected with Francisella tularensis

Project description:Differential expression in human peripheral blood monocytes between F. novicida-infected and uninfected, and between Francisella tularensis tularensis isolate Schu S4 and uninfected. The goal was to examine genomewide transcriptional reponses to these two strains, and identify differentially-regulated genes that may help explain the virulence of Schu S4. Experiment Overall Design: Human monocytes were infected with the Schu S4 isolate of Francisella tularensis tularensis (n=4), with F. tularensis subspecies novicida isolate U112 (n=4) or were left uninfected (n=6). Gene expression values were calculated using the gcrma package in R and BioConductor, and limma to identify differentially expressed genes. Submitted here are expression values calculated using R 2.7.1 and BioConductor 2.2 (FreeBSD/amd64) but the original were done using R 2.6.1 and BioConductor 2.1 (FreeBSD/amd64). Twelve other chips were pooled with these 14 for preprocessing.

2008-10-25 | E-GEOD-12108 | biostudies-arrayexpress

Francisella tularensis subsp. novicida D9876

Project description:Francisella tularensis subsp. novicida D9876 Genome sequencing

| PRJNA235892 | ENA

Francisella tularensis subsp. novicida FSC160

Project description:Francisella tularensis subsp. novicida FSC160 Genome sequencing

| PRJNA73385 | ENA

Francisella tularensis subsp. novicida FSC159

Project description:Francisella tularensis subsp. novicida FSC159 Genome sequencing

| PRJNA73383 | ENA

Francisella tularensis subsp. novicida F6168

Project description:Francisella tularensis subsp. novicida F6168 Genome sequencing