Project description:To annotate estrogen-regulated eRNAs in MCF-7 breast cancer cells, we used precision nuclear run-on and sequencing of capped RNA (PRO-cap) to determine the transcription start sites of eRNAs.
Project description:To annotate estrogen-regulated eRNAs in MCF-7 breast cancer cells, we used RNA-sequencing of polyA-depleted and polyA-enriched RNA fractions to assemble the gene bodies of eRNAs.
Project description:Mapping of active transcription start sites using PRO-cap in whole-embryo at 3-4h and 6-8h AEL. Two biological replicates were performed at each time point.