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Changes in chromatin accessibility when expressing two sets of t(6;11) fusion proteins

ABSTRACT: Changes in chromatin accessibility when expressing two sets of t(6;11) fusion proteins

PROVIDER: PRJNA732711 | ENA |

REPOSITORIES: ENA

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Changes in chromatin accessibility when expressing two sets of t(6;11) fusion proteins

Project description:We have cloned two sets of t(6;11) fusion genes with breakpoints in the majorand minor BCR of the MLL/KMT2A gene. Both setsof fusion genes were stably transfected alone or in combiantion to analyze their molecular power

2021-05-26 | GSE175558 | GEO

Changes in gene expression when expressing two sets of t(6;11) fusion proteins

Project description:Changes in gene expression when expressing two sets of t(6;11) fusion proteins

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Changes in gene expression when expressing two sets of t(6;11) fusion proteins

2021-05-27 | GSE175573 | GEO

Changes in chromatin accessibility over time in cells expressing t(4;11) fusion proteins

Project description:Changes in chromatin accessibility over time in cells expressing t(4;11) fusion proteins

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Changes in chromatin accessibility over time in cells expressing t(4;11) fusion proteins [II]

Project description:Changes in chromatin accessibility over time in cells expressing t(4;11) fusion proteins [II]

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Changes in chromatin accessibility over time in cells expressing t(4;11) fusion proteins [I]

Project description:Changes in chromatin accessibility over time in cells expressing t(4;11) fusion proteins [I]

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Changes in chromatin accessibility over time in cells expressing t(4;11) fusion proteins [I]

Project description:We have used a constitutive MLL-AF4 fusion gene together with a inducible AF4-MLL fusion gene to investigate the power of theses fusion proteins. All transgenes were stably or transiently transfected.

2021-06-22 | GSE178567 | GEO

Changes in chromatin accessibility over time in cells expressing t(4;11) fusion proteins [II]

Project description:We have used a combination of constitutive and inducible MLL-AF4 and AF4-MLL fusion genes to investigate the power of theses fusion proteins. All transgenes were stably or transiently transfected. Here, we compared 48 induction of MLL-AF4 or AF4-MLL (day 3) and looked for persistance at days 28. This was compared to the situation with constitutive MLL-AF4 with 48 inductionn of AF4-MLL at day 3 and day 28.

2021-06-22 | GSE178568 | GEO

Changes in gene expression over time in cells expressing t(4;11) fusion proteins

Project description:Changes in gene expression over time in cells expressing t(4;11) fusion proteins

| PRJNA739158 | ENA

iCLIP of GFP fusion proteins

Project description:HeLa cells were cultured in DMEM, supplemented with 10% (v/v) FCS and penicillin/streptomycin under 5% CO2 at 37C. For iCLIP, HeLa cells expressing GFP fusion proteins were induced with doxycycline to adjust the level of recombinant protein to the level of the endogenous counterpart and irradiated with 150 mJ/cm2 UV light (254 nm). The iCLIP cDNA libraries were sequenced with 50 bp on an Illumina HiSeq 2000 instrument. RNASeq was performed as a control with 50 bp paired-end on an Illumina HiSeq 2000 instrument.

2016-07-28 | E-MTAB-4215 | biostudies-arrayexpress

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