Project description:Jurkat T cells have been lysed after exposure to 15 minutes of 9g in a ground-based pipette centrifuge. The RNA samples were parallely analyzed with RNA-Seq and microarray transcriptomics.

2021-08-31 | GSE175798 | GEO

Comparative Transcriptomics on cellular reactions to short-term hypergravity [RNA-Seq]

2021-08-31 | GSE175799 | GEO

Short-term hypergravity effects on Jurkat T cells

Project description:Jurkat T cells have been exposed to 9g hypergravity in a custom-built pipette centrifuge for different times (GBF2021). Additionally, Jurkat T cells have been exposed to 300g for 5 minutes in a standard benchtop centrifuge, and waited for different times until adding lysis buffer. For comparison, Jurkat T cells have been exposed to 5 minutes of 42°C.

2023-02-07 | GSE221579 | GEO

Jurkat T cell exposure to short hypergravity on a ground-based facility

Project description:Jurkat T cells have been lysed after exposure to 3 or 15 minutes of 9g hypergravity in a ground-based pipette centrifuge. The RNA samples were analyzed with RNA-Seq transcriptomics.

2022-07-27 | GSE190484 | GEO

Evaluation of the effects of microgravity and hypergravity on hepatic stellate cells

Project description:In this study, we evaluated cell proliferation and gene expression of hepatic stellate cells (HSCs) in simulated microgravity (SMG) and hypergravity (5 G).

2021-06-25 | GSE178831 | GEO

Short enzymatic reactions in proteomics studies

Project description:SKBR3 breast cancer cell extracts were digested with trypsin (or LysC) on short time scales (7min, 15min, 30min, 1h, and 18h), and the results were compared to overnight digestion protocols.

2018-09-24 | PXD010306 | Pride

Transcriptomic response of Drosophila melanogaster pupae developed in hypergravity

Project description:Physical forces greatly influence the growth and function of an organism. Altered gravity can perturb normal development and induce corresponding changes in gene expression. Understanding this relationship between the physical and biological realms is important for NASA’s space travel goals. We use combined RNA-Seq and qRT-PCR to profile changes in early Drosophila melanogaster pupae exposed to chronic hypergravity (3 g, three times Earth’s gravity) to highlight gravity-dependent pathways and gene products. Robust transcriptional response was evident among the pupae developed in a hypergravity environment compared to control. 1,513 genes showed significantly (p < 0.05) altered gene expression in the 3 g samples. These findings were supported with qRT-PCR data. Major biological processes affected include ion transport, redox homeostasis, immune and humoral stress response, proteolysis, and cuticle development.

2016-09-19 | GSE80323 | GEO

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