Microcebus arnholdi
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ABSTRACT:
PROVIDER: PRJNA734403 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Double-digest Restriction-site associated DNA sequencing (ddRAD-seq) of wild mouse lemurs (genus Microcebus)
| PRJNA318921 | ENA
Project description:Microcebus sp. JM-2020 Genome sequencing
| PRJNA632451 | ENA
Project description:Gene expression data from temporal cortex of young adult, old and AD-like Microcebus murinus
| PRJNA127303 | ENA
Project description:Aging is the primary risk factor of neurodegenerative disorders such as Alzheimer's disease (AD). However, the molecular events occurring during brain aging are extremely complex and still largely unknown. For a better understanding of these age-associated modifications, animal models as close as possible to humans are needed. We thus analyzed the transcriptome of the temporal cortex of the primate Microcebus murinus using human oligonucleotide microarrays (Affymetrix). Gene expression profiles were assessed in the temporal cortex of 6 young adults, 10 healthy old animals and 2 old, "AD-like" animals that presented b-amyloid plaques and cortical atrophy, which are pathognomonic signs of AD in humans. Gene expression data of the 14,911 genes that were detected in at least 3 samples were analyzed. By SAM (significance analysis of microarrays), we identified 47 genes that discriminated young from healthy old and "AD-like" animals. These findings were confirmed by principal component analysis (PCA). ANOVA of the expression data from the three groups identified 695 genes (including the 47 genes previously identified by SAM and PCA) with significant changes of expression in old and "AD-like" in comparison to young animals. About one third of these genes showed similar changes of expression in healthy aging and in M-bM-^@M-^\AD-likeM-bM-^@M-^] animals, whereas more than two thirds showed opposite changes in these two groups in comparison to young animals. Hierarchical clustering analysis of the 695 markers indicated that each group had distinct expression profiles which characterized each group, especially the "AD-like" group. Functional categorization showed that most of the genes that were up-regulated in healthy old and down-regulated in "AD-like" animals belonged to metabolic pathways, particularly protein synthesis. These data suggest the existence of compensatory mechanisms during physiological brain aging that disappear in M-bM-^@M-^\AD-likeM-bM-^@M-^] animals. These results open the way to new exploration of physiological and M-bM-^@M-^\AD-likeM-bM-^@M-^] aging in primates. Microcebus murinus were divided in 3 groups: the first group included 6 young adults (4 females and 2 males), the second included 10 healthy old animals (7 females and 3 males) and the third one was composed by 2 "AD-like" old females. Since Microcebus murinus microarrays do not exist, we decided to use Affymetrix human genome chips (HG U133 plus 2), since studies have illustrated the feasibility of detecting non-human primate brain transcripts using human genome chips.
2011-04-01 | E-GEOD-21779 | biostudies-arrayexpress
Project description:Gene expression changes in the midbrain of Microcebus murinus induced by Canine adenovirus type 2 (CAV-2)
| PRJNA398483 | ENA
Project description:Gene expression changes in the striatum of Microcebus murinus induced by Canine adenovirus type 2 (CAV-2)
| PRJNA398267 | ENA
Project description:Gene expression changes in the frontal cortex of Microcebus murinus induced by Canine adenovirus type 2 (CAV-2)
| PRJNA398482 | ENA
Project description:Mouse lemurs are basal primates that rely on chemo- and acoustic signalling for social interactions in their dispersed social systems. We examined the urinary protein content of two mouse lemurs species, within and outside the breeding season, to assess candidates used in species discrimination, reproductive or competitive communication. Urine from Microcebus murinus and Microcebus lehilahytsara contain a predominant 10kDa protein, expressed in both species by some, but not all, males during the breeding season, but at very low levels by females. Mass spectrometry of the intact proteins confirmed the protein mass and revealed a 30 Da mass difference between proteins from the two species. Tandem mass spectrometry after digestion with three proteases and sequencing de novo defined the complete protein sequence and located an Ala/Thr difference between the two species that explained the 30 Da mass difference. The protein (mature form: 87 amino acids) is an atypical member of the whey acidic protein family (WFDC12). Seasonal excretion of this protein, species difference and male-specific expression during the breeding season suggest that it may have a function in intra- and/or intersexual chemical signalling in the context of reproduction, and could be a cue for sexual selection and species recognition.
2017-02-13 | PXD005196 | Pride