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Next Generation Sequencing Facilitates Quantitative Analysis of differentially expressed genes between wild type and the fiber mutant Xuzhou142fl

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of differentially expressed genes between wild type and the fiber mutant Xuzhou142fl

PROVIDER: PRJNA736045 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing Facilitates Quantitative Analysis of differentially expressed genes between wild type and the fiber mutant Xuzhou142fl

Project description:Tetraploid cotton plants serve as prime natural fiber source for the textile industry. Although various omics studies have revealed molecular basis for fiber development, a better understanding of transcriptional regulation mechanism regulating lint fiber initiation is necessary to meet global natural fiber demand. Here, we aimed to perform transcriptome sequencing to identify DEGs (differentially expressed genes) in ovules of the cotton variety Xu142 and its fibreless mutant Xu142fl during early lint fiber initiation period. Totally, 5516 DEGs including 1840 upregulated and 3676 downregulated were identified. GO enrichment analysis revealed that the downregulated DEGs mainly associated with biological processes such as transcription related biosynthesis and metabolism, organic cyclic compound biosynthesis and metabolism, photosynthesis, and plant cell wall organization, with molecular functions involving transcription related binding, organic cyclic compound binding, and dioxygenase activity, while the upregulated DEGs were associated with DNA replication and phospholipid biosynthetic related processes. Among the 490 DEGs annotated as transcription factor genes 86.5% were downregulated in the mutant including the Malvaceae-specific MMLs, expression patterns of which were confirmed during the central period of lint fiber initiation. Investigation of the 20 genes enriched in the cell wall organization revealed that 17 were EXPA coding genes. Overall, we suggest that lint fiber initiation is a complicated process involving cooperation of multiple transcription factor families, which ultimately lead to the reorganization of the cell wall and terminated cell division of the differentiating fiber initials.

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Genes differentially expressed in fiber 1 day post anthesis (dpa)

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