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RNA-sequencing uncovers transcriptomic changes induced by a multiblock thermogelling polymer on healthy and disease-modeled retinal pigment epithelial cells

ABSTRACT: RNA-sequencing uncovers transcriptomic changes induced by a multiblock thermogelling polymer on healthy and disease-modeled retinal pigment epithelial cells

PROVIDER: PRJNA736472 | ENA |

REPOSITORIES: ENA

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RNA-sequencing uncovers transcriptomic changes induced by a multiblock thermogelling polymer on healthy and disease-modeled retinal pigment epithelial cells

Project description:Human embryonic stem-cell derived retinal pigment epithelial cells were co-stimulated with TNF-α and TGF-β1 to model a retinal scarring complication, proliferative vitreoretinopathy. Thermogelling polymer prevented this scarring complication. To understand the polymer-cell interaction and ultimately the mechanism underlying it's anti-scarring activity, RNA-Seq was performed.

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RNA-sequencing uncovers transcriptomes of human diseased, healthy, and disease-modeled retinal pigment epithelial cells.

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RNA-sequencing uncovers transcriptomic changes in retinal tissues after vitreous substitution with a thermogelling polymer

Project description:We developed a thermogelling polymer with intrinsic anti-scarring properties on retinal cells in-vitro. To study its mechanism of action in-vivo, this thermogelling polymer was injected into the rabbit eye as a vitreous substitute and the retinal tissues were harvested for RNA-sequencing to understand transcriptomic changes induced by the polymer.

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2015-07-24 | E-MTAB-3137 | biostudies-arrayexpress

RNA-sequencing uncovers transcriptomic changes in retinal tissues after vitreous substitution with a thermogelling polymer

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ChIP-seq of RNA polymerase II, transcription factors and histone modification in retinal pigment epithelial cells

Project description:Retinal pigment epithelial cells are critical for eye function and loss of cell function is linked to age-related blindness. Relatively little is known about the transcriptional regulatory networks in these cells. The datasets presented here are ChIP-seq experiments for RNA polymerase II , transcription factors and histone modifications in human retinal pigment epithelial cells. ChIP-Seq for transcription factors, RNA polymerase, histone modifications and CTCF in retinal pigment epithelial cells

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cryo-EM study of bovine retinal pigment epithelium cell lysate

Project description:This is the proteomic analysis data of bovine retinal pigment epithelium cell lysate subjected to cryo-EM study.

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Oxidative stress alters transcript localization of disease-causing genes in the retinal pigment epithelium

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Retinal pigment epithelial cells suppress interleukin-17-producing T-helper 17 cells

Project description:T cells that encounter cultured ocular pigment epithelial cells in vitro are inhibited from undergoing T cell receptor-triggered activation. Because retinal pigment epithelial (RPE) cells are able to suppress T-cell activation, we studied whether RPE cells could suppress cytokine production by activated T helper (Th) cells. In this study we showed that primary cultured RPE cells greatly suppressed activation of bystander CD4+ T cells in vitro, especially the cytokine production by the target T helper cells (Th1 cells, Th2 cells, Th17 cells, but not Th3 cells). Cultured RPE cells and RPE-supernatants significantly suppressed IL-17 producing CD4+ T cells, and RPE cells fully suppressed polarized Th17 cell lines that induced by recombinant proteins, IL-6 and TGFb2. Moreover, RPE cells failed to suppress IL-17 producing T cells in the presence of rIL-6. In addition, Th17 cells exposed to RPE were suppressed via TGFb, which produce RPE cells. These results indicate that retinal PE cells have immunosuppressive capacity in order to inhibit Th17-type effector T cells. Thus, ocular resident cells play a role in establishing immune regulation in the eye. Retinal pigment epithelium suppresses Th17 cells

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