Transcriptomic analysis of embryonic hearts with conditional deletion of Wt1
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ABSTRACT:
PROVIDER: PRJNA737788 | ENA |
REPOSITORIES: ENA
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2014-05-06 | GSE46664 | GEO
Project description:We are characterizing global expression in the whole kidney at developmental stage E18.5, where we have used three Cre strains that result in knock out Wt1 in different cell types during renal development. Here the purpose is to identify the developmental stage at which the loss of Wt1 impacts kidney development, specifically in the context of a degree of disruption capable of ending in pediatric kidney cancers. Total RNA was isolated from whole kidneys of E18.5 mouse embryos following a variety of conditional knockouts of Wt1, plus appropriate control animals.
2015-07-27 | E-GEOD-70892 | biostudies-arrayexpress
Project description:The Wilms tumor gene, Wt1, is specifically expressed in Sertoli cells (SCs) which support spermatogenesis. Germ cell loss was found in Wt1 knockout mice. In vitro studies demonstrated that Wt1 was essential for cell polarity maintenance in SCs. We used Digital Gene Expression Tag Profiling to detail the global programme of gene expression after Wt1 deletion in sertoli cell and identified 710 gene expression level change more than 2 times. Digital Gene Expression Tag Profiling analysis were performed using mRNA from control and Wt1-deficient Primary Sertoli cells, every group primare sertoli cell were from more than 30 mice.
2014-05-06 | E-GEOD-46664 | biostudies-arrayexpress
Project description:The RNA-sequence analysis of cardiomyocyte-specific deletion of STAT3 mice hearts showed that comparing with WT mice hearts, the STAT3cKO mice hearts showed reduced cardiac function by affecting some key pathways.
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Project description:K-ras is one of the most frequently mutated human oncogenes. Activation of K-ras can lead to either senescence or proliferation in primary cells. The precise mechanism governing these distinct outcomes remains unclear. Here we utilized a loss-of-function screen to assess the role of specific genes identified as potential key regulators of K-ras driven oncogenesis. Using this approach, we identify the transcription factor Wt1 as an inhibitor of senescence in primary cells expressing oncogenic K-ras. Deletion or suppression of Wt1 expression leads to senescence of primary cells expressing oncogenic K-ras under the control of the native promotor at physiological levels, but has no effect on cells expressing wild-type K-ras. Wt1 contributes to K-ras driven lung tumorigenesis in vivo and loss of Wt1 is specifically deleterious to human lung cancer cell lines that are dependent on oncogenic K-ras. Taken together, these observations reveal a novel role for Wt1 as a key regulator of the complex genetic network required for the oncogenic effect of the small GTPase K-ras. We compare the expression profiles of Wild type, K-ras mutant, Wt1-null, and double K-ras mutant/Wt1-null mouse embryonic fibroblasts (MEFs). The study provides insights into the transcriptional role of Wt1 in the context of oncogenic K-ras.
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Project description:We generated the cardiac-specific knockout of Trbp (Trbp-cKO) in mice. We profiled the transcriptome in both wild-type and Trbp-cKO hearts, and found numerous genes were deregulated by deletion of Trbp. We also profiled miRNA expression both wild-type and Trbp-cKO hearts, and found expression of a subset of miRNA species was altered in Trbp-cKO hearts.
2015-04-27 | GSE67658 | GEO
Project description:We generated the cardiac-specific knockout of Trbp (Trbp-cKO) in mice. We profiled the transcriptome in both wild-type and Trbp-cKO hearts, and found numerous genes were deregulated by deletion of Trbp. We also profiled miRNA expression both wild-type and Trbp-cKO hearts, and found expression of a subset of miRNA species was altered in Trbp-cKO hearts. Examine expression of mRNAs and miRNAs in wild-type and Trbp-cKO hearts
2015-04-27 | E-GEOD-67658 | biostudies-arrayexpress
Project description:Purpose: Study the differentially expressed genes in the hippocampus of mice, comparing Wt1∆ mice with their control wild type littermates in basal conditions (naïve untrained animals). Methods: Forebrain-specific deletion of Wt1 was achieved by crossing animals homozygous for the conditional Wt1 knockout allele (Wt1fl/fl) with a transgenic line, Camk2a-Cre, (B6.Cg-Tg(Camk2a-cre)T29-1Stl/J; Jackson Lab: http://jaxmice.jax.org/strain/005359.html). Expression of Cre recombinase resulted in the in-frame deletion of exons 8 and 9 and generated a truncated allele encoding a shortened non functional WT1 protein lacking zinc fingers 2 and 3 in their forebrain (starting at P21). Brains of Wt1∆ mice (n=2) and their control littermates (wild type; n=2) were removed, dorsal hippocampi were dissected and used for library preparation. The seq library was prepared using TruSeq RNA Sample Prep Kit v2 protocol (Illumina, CA, USA). For the RNA-Seq data analysis Tophat 2.0.13, bowtie 2.1.0 , samtool 0.1.7 and cufflinks 1.3.0 were used. The rn5-bowtie2 index was generated with the command 'bowtie2-build rn5.fa rn5'. The 'rn5.fa'-file was downloaded from the UCSC genome browser. The mm10-bowtie2 index was downloaded from http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml. RefSeq geneTracks and GTF-files for the rn5 and mm10 genome assembly were downloaded from UCSC genome browser. Common gene ids in the GTF-files were matched to individual transcript_ids using the corresponding official symbols obtained from the geneTracks files. Conclusions: Our study illustrates the nature of the different transcripts in Wt1∆ mice compared to their control wild type littermates at basal state (untrained). This experiment allowed us to identify wich genes are modulated in the hippocampus by the transcription fator WT1.
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Project description:We performed RNA-seq 72 h after acute deletion of Smarca4 in a line of conditional knockout mouse embryonic stem cells to examine altered gene expression.
2016-10-21 | GSE88967 | GEO