Project description:We report the genetic plasticity of Sendai virus and mumps virus. We introduced insertional mutation in the virus genome and checked fitness by comparing distribution of mutans in passage 1 and passage 2.
Project description:We evaluate the individual growth of 8 mutants of Sendai virus in the competitive condition under the cultured cells. We also evalate the growth of 6 mutants of mumps virus in the similar competitive condition.
Project description:Mumps virus (MuV) is the etiological agent of mumps, a disease characterized by painful swelling of the parotid glands and often accompanied by severe complications. To understand the molecular mechanism of MuV infection, a functional analysis of the involved host factors is required. However, little is known about the host factors involved in MuV infection, especially those involved in the late stage of infection. In this project, we introduced the N-terminal and C-terminal fragments of split-TurboID into the cytoplasmic domains of hemagglutinin-neuraminidase and fusion proteins, respectively, and confirmed reconstitution and functional ligase activity of split-TurboID. Following affinity purification and mass spectrometry, 641 proteins were identified as being significantly enriched in the split-TurboID-expressing cells.
Project description:Recombinant Mumps viruses grown on Vero cells (Cercopithecus aethiops). Detection and quantification of defective viral genomes (DVGs) in high throughput sequencing data using DVG-profiler, a novel post sequence alignment processing algorithm.
