Project description:Water transport is required for photosynthesis; thus plant vascular development must proceed such that water demands can be met during formation of new organs. Precise regulation of the balance between cell proliferation and differentiation in the cambial meristem defines formation of xylem and phloem, which constitute the vascular tissues. The TDIF-PXY/TDR signalling pathway is central to this process1. Peptide ligand, TDIF, encoded by CLE41 and CLE44 2, activates PXY/TDR receptors to maintain proliferative cambium. Cambium cells differentiate to xylem or phloem in the absence of active TDIF-PXY/TDR complexes. Vascular development is stimulated by light. After gemination, light induces the establishment of photoautotrophic growth via photosynthesis. Consequently, the differentiation of vascular cells must occur to favour the transport of water and minerals from the soil to the green tissues and newly developing organs. Plants perceive light through the action of photoreceptors, which modulate the activity of the transcription factors that orchestrate development. Despite the association between light signalling and vascular development, molecular mechanisms linking the two are unknown. Our work shows that vascular differentiation is inhibited in the dark by a mechanism that depends on PIF transcription factors. The dark accumulation of PIFs is necessary for CLE44 induction and thus maintenance of undifferentiated vasculature. In illuminated environments, PIF inactivation by photoreceptors causes a decrease in CLE44 expression. CLE44 reduction, in turn, leads to reduced PXY/TDR signalling which induces the xylem differentiation required to fulfil the water demands associated with photoautotrophic development.
Project description:This SuperSeries is composed of the following subset Series: GSE37678: cDNA Microarray 1: Compression Xylem vs. Opposite Xylem GSE37736: cDNA Microarray 2: Compression Xylem vs. Opposite Xylem Refer to individual Series
Project description:We take the two year old plant for sampling. Use the Affymetrix poplar gene chip to elucidate the gene functions and mechanisms in Populus tomentosa newly formed developing xylem and lignified xylem. We used microarrays to detail the global programme of gene expression in newly formed developing xylem and lignified xylem.
Project description:Downstream genes of PtVNS genes were explored with inducible expression system using glucocorticoid receptor (GR). Transgenic poplar plants expressing 35S:AtVND7-VP16-GR were treated with dexamethazone (DEX). A number of genes related to the formation of xylem vessels were induced by DEX.
Project description:We take the two year old plant for sampling. Use the Affymetrix poplar gene chip to elucidate the gene functions and mechanisms in Populus tomentosa newly formed developing xylem and lignified xylem. We used microarrays to detail the global programme of gene expression in newly formed developing xylem and lignified xylem. Populus tomentosa newly formed developing xylem and lignified xylem were taken for RNA extraction and hybridization on Affymetrix microarrays. CB2009304-A and CB2009304-B from newly formed developing xylem, CB2009304-G and CB2009304-H from lignified xylem.
Project description:12plex_poplar_2012-01 - gravitropic stimulation / isotropic light - The experiments were designed to identify genes responding to the gravitropic stimulus in the xylem of poplar stem independently of phototropic responses. - young poplar trees were staked one week before use. For the experiment, the plants were placed in an isotropic light environment and were tited at 35° during 30 min. Control plants were kept straight in the isotropic light device. Xylem samples were collected from the upper and lower face of the stems. 4 biological replicates were made (4 controls and 4 tilted plants). RNA was extracted from each xylem sample. cDNA was synthetized. The discovery of genes whose expression is modulated by tilting was performed by a microarray approach.
Project description:The Arabidopsis hypocotyl is an excellent model for understanding radial growth in plants. Division of the cambial cells and their subsequent differentiation into xylem and phloem drives radial expansion of the hypocotyl. Following the transition to reproductive growth, a phase change occurs in the Arabidopsis hypocotyl. During this second phase, the relative rate of xylem production is dramatically increased compared to that of phloem and xylem fibres containing thick secondary cell walls also form, which results in the production of xylem tissue comparable to the wood of trees. Abscisic acid (ABA) is a phytohormone known to have a major role in various plant processes, including in the response to changes in environmental conditions and in the promotion of seed dormancy. Using two different genetic backgrounds and different environmental conditions, we identified a set of core of transcriptional changes associated with the switch to the second phase of growth in the hypocotyl. ABA signalling pathways were identified as being as significantly over-represented in this set of core genes. Reverse genetic analysis demonstrated that mutants defective in ABA-biosynthesis enzymes exhibited significantly delayed fibre production without affecting the xylem:phloem ratio. The altered morphology is also reflected at the transcript level, with a reduced expression of marker genes associated with fibre formation in aba1 mutants. The application of exogenous ABA to the mutant rescued the phenotype, restoring fibre differentiation to wild-type levels. Taken together the data reveals an essential role for ABA in the regulation of fibre formation.
Project description:The Arabidopsis hypocotyl is an excellent model for understanding radial growth in plants. Division of the cambial cells and their subsequent differentiation into xylem and phloem drives radial expansion of the hypocotyl. Following the transition to reproductive growth, a phase change occurs in the Arabidopsis hypocotyl. During this second phase, the relative rate of xylem production is dramatically increased compared to that of phloem and xylem fibres containing thick secondary cell walls also form, which results in the production of xylem tissue comparable to the wood of trees. Abscisic acid (ABA) is a phytohormone known to have a major role in various plant processes, including in the response to changes in environmental conditions and in the promotion of seed dormancy. Using two different genetic backgrounds and different environmental conditions, we identified a set of core of transcriptional changes associated with the switch to the second phase of growth in the hypocotyl. ABA signalling pathways were identified as being as significantly over-represented in this set of core genes. Reverse genetic analysis demonstrated that mutants defective in ABA-biosynthesis enzymes exhibited significantly delayed fibre production without affecting the xylem:phloem ratio. The altered morphology is also reflected at the transcript level, with a reduced expression of marker genes associated with fibre formation in aba1 mutants. The application of exogenous ABA to the mutant rescued the phenotype, restoring fibre differentiation to wild-type levels. Taken together the data reveals an essential role for ABA in the regulation of fibre formation. We used microarrays to probe transcripome changes I Arabidopsis hypocotyls following transition from phase I to phase II
Project description:The hemera (hmr) mutant was identified as the first photomorphogenetic mutant with the combination of long hypocotyl and albino phenotypes in the light. Phytochrome-Interacting bHLH transcription Factors (PIFs), which are repressors of photomorphogenesis accumulate in darkness and are degraded in the light in a phytochrome-dependent manner. Two PIFs, PIF1 and PIF3 accumulated in the light in hmr mutants. In order to determine the gene expression of PIF-dependent genes in hmr mutants in the light, we have performed whole-genome expression analysis on two hmr mutants: a null allele, hmr-5; and a weak allele, hmr-22.