Project description:We profiled the single-cell transcriptomes of 13,289 peripheral blood mononuclear cells isolated at three longitudinal stages from two severe COVID-19 patients treated with Tocilizumab. The raw sequencing data can be obtained from the Genome Sequence Archive for Human (GSA-Human) at https://bigd.big.ac.cn/gsa-human/browse/HRA000172 .

2020-07-21 | GSE150861 | GEO

Homo sapiens

Project description:Human Peripheral blood mononuclear cells (PBMCs): Control vs. Kashin-Beck Disease

| PRJNA255333 | ENA

Selective inhibition of Homo sapiens M1 macrophages where SP140 mRNA is inhibited using siRNA

Project description:As part of our study in understanding the role of SP140 in inflammatory pathways in macrophages, we inhibited SP140 mRNA using siRNA. Peripheral blood mononuclear cells (PBMCs) were obtained from whole blood of healthy donors (from Sanquin Institute Amsterdam or from GSK Stevenage Blood Donation Unit) by Ficoll density gradient (Invitrogen). CD14+ monocytes were positively selected from PBMCs using CD14 Microbeads according to the manufacturer’s instructions (Miltenyi Biotec). CD14+ cells were differentiated with 20 ng/mL of macrophage colony-stimulating factor (M-CSF) (R&D systems) for 3 days followed by 3 days of polarization into classically activated (inflammatory) M1 macrophages (100 ng/mL IFN-γ; R&D systems). M1 macrophages were transfected with siGENOME human smartpool SP140 siRNA or non-targeting scrambled siRNA for 48h with DharmaFECT™ transfection reagents according to manufacturer’s protocol (Dharmacon). The cells were left unstimulated or stimulated with 100 ng/mL LPS (E. coli 0111:B4; Sigma) for 4h (for qPCR) or 24h (for Elisa). The cells were lysed (ISOLATE II RNA Lysis Buffer RLY-Bioline) for RNA extraction.150 ng total RNA was labelled using the cRNA labelling kit for Illumina BeadArrays (Ambion) and hybridized with Ref8v3 BeadArrays (Illumina). Arrays were scanned on a BeadArray 500GX scanner and data were normalized using quantile normalization with background subtraction (GenomeStudio software; Illumina). This submission only contains processed data

2022-07-22 | E-MTAB-12011 | biostudies-arrayexpress

Homo sapiens

Project description:Transcriptome analysis of human peripheral blood mononuclear cell (PBMC)

| PRJNA422156 | ENA

Homo sapiens

Project description:human Growth Hormone (GH) responsive genes in peripheral blood mononuclear cells (PBMC)

| PRJNA137073 | ENA

Homo sapiens

Project description:Ionizing Radiation Induced Gene Expression Alterations in Human Peripheral Blood Mononuclear Cells

| PRJNA241426 | ENA