Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study was to obtain the trasncriptome of Ago2_KO mESCs to compare it with the transcriptome of WT mESCs before and after XEN conversion (deposit separately).
Project description:Bcl3 is expressed in mESCs. ChIP-seq was used to identify its binding pattern and target genes. ChIP of Bcl3 in mESCs under conventional culture condition
Project description:In order to identify direct microRNA targets in mouse embryonic stem cells (mESCs), we knocked out microRNA effector genes, profiled their transcriptomes and integrated the data with additional published OMICs data sets. To validate the analysis, we generated miR-290-295 KO mutant cell lines, assessed the transcriptome profile and compared the observed misregulation to the predictions of the analysis.
Project description:The goal was to identfiy the transcriptomics changes in mESCs upon Ago1 depletion. WT duplicate samples that are experimentally comparable, have been uploaded to GEO previously (GSM2082855, GSM2082856)
Project description:We treated mESCs with 50mM lactate to examine its impact on mESC transcriptome. Interestingly, we found that lactate supplementation activated both germline genes and cleavage embryo genes, indicating that lactate supplementation expands transcriptional network in mouse ESCs.
Project description:Genome-wide occupancy of biotinylated Jmjd2b, Jmjd2c from mESCs, as well as occupancy of selected factors and histone marks from wild-type mESCs, Anti-GFP KD, Jmj2b KD and Jmjd2c KD mESCs genome To identify genome-wide binding target sites of Jmjd2b and Jmjd2c in the mESCs genome, and genome-wide binding sites for selected factors and histone marks from Anti-GFP KD, Jmjd2b KD and Jmjd2c KD mESCs
Project description:Genome-wide occupancy of biotinylated Jmjd2b, Jmjd2c from mESCs, as well as occupancy of selected factors and histone marks from wild-type mESCs, Anti-GFP KD, Jmj2b KD and Jmjd2c KD mESCs genome
