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Whole transcriptome sequencing of HT22 immortalized mouse hippocampal cells treated with AA147 or veh for 16 h

ABSTRACT: Whole transcriptome sequencing of HT22 immortalized mouse hippocampal cells treated with AA147 or veh for 16 h

PROVIDER: PRJNA741680 | ENA |

REPOSITORIES: ENA

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Whole transcriptome sequencing of HT22 immortalized mouse hippocampal cells treated with AA147 or veh for 16 h

Project description:Purpose: Define transcriptional remodeling associated with AA147 treatment (10uM) for 16 h in a mouse neuronal cell line. Methods: A minimum of 27M PE100 reads were generated for triplicates of vehicle vs. AA147 treated HT22 cells. Alignment of reads was performed using DNAstar Lasergene SeqManPro to the mouse genome GRCm39 assembly. Read counts generated by ArrayStar 12.2 QSeq package. Differential expression analysis performed using DESeq2. Results: We mapped between 27-32 M sequence reads per sample to the mouse genome and identified 16,473 transcripts with counts greater than 10. Of these, we identified 2,742 genes with significantly different expression between vehicle and AA147 treated cells. Conclusions: We observed substantial upregulation of prolactin and glutathione transferase genes, and upregulation of NRF2-associated pathway upregulation.

2021-12-06 | GSE178964 | GEO

Expression data from J147-treated HT22 cells compared to untreated HT22 cells

Project description:The Neurotrophic Compound J147 Reverses Cognitive Impairment In Aged Alzheimer's Disease We used GeneChipM-BM-. Mouse Genome 430 2.0 Arrays to probe global gene expression changes when adding J147 compound to HT22 cells and compared to untreated cells. The hippocampal cell line HT22 were treated with the J147 compound for 1 hour and compared to HT22 treated only with vehicle (DMSO) for 1 hour. The gene expression profile from the J147-treated HT22 cells (1 hour exposure) was compared to HT22 cells treated with vehicle only (DMSO) for 1 hour. The DMSO/vehicle treated cells serve as the control and are referred to as untreated with drug.

2013-03-28 | E-GEOD-45534 | biostudies-arrayexpress

Chromatin profiling in HT22 hippocampal cells

Project description:Chromatin profiling in HT22 hippocampal cells

| PRJNA521221 | ENA

Chromatin Immunoprecipitation analysis of mouse hippocampal neuron HT22 cells

Project description:We used H4K5bu and H4K5ac antibodies to specifically enrich mouse hippocampal neuronal cells (HT22) before and after BHBA administration. We found that the acetylation and butylation of histone H4K5 play a key role in the neuroprotection of BHBA.

2021-04-09 | GSE171743 | GEO

Expression data from J147-treated HT22 cells compared to untreated HT22 cells

Project description:The Neurotrophic Compound J147 Reverses Cognitive Impairment In Aged Alzheimer's Disease We used GeneChip® Mouse Genome 430 2.0 Arrays to probe global gene expression changes when adding J147 compound to HT22 cells and compared to untreated cells. The hippocampal cell line HT22 were treated with the J147 compound for 1 hour and compared to HT22 treated only with vehicle (DMSO) for 1 hour.

2013-03-28 | GSE45534 | GEO

Effects of NMDAR on mouse hippocampal neuron (HT22) mRNA and lncRNA expression during hypoxia/reoxygenation injury

Project description:To investigate the changes of the transcriptome in neuronal cells under the condition of NMDAR hypofunction and H/R injury, we compared the changes of mRNA and lncRNA expression levels in mouse hippocampal neuron HT22 cell with or without NMDAR knockdown and H/R stimulation by RNA sequencing.

2022-10-10 | GSE214716 | GEO

Chromatin Immunoprecipitation analysis of mouse hippocampal neuron HT22 cells

Project description:Chromatin Immunoprecipitation analysis of mouse hippocampal neuron HT22 cells

| PRJNA720695 | ENA

Genome-wide analysis of gene expression in wild-type and KMT2D-deficient HT22 cells

Project description:We conducted genome wide transcription profiling by RNA-seq in a mouse hippocampal cell line (HT22), comparing cells with CRISPR-Cas9 mediated deletion of the KMT2D enzymatic SET methyltransferase domain to the parental cell line

2019-02-07 | GSE126165 | GEO

The mRNAs alteration induced by Neat1 knockdown in HT22 cells

Project description:We knocked down the lncRNA-Neat1 by RNA interference in HT22 cells, which were immortalized from the mice hippocampal neurons. Then we measured the mRNA alteration induced by Neat1 knockdown through the next generation sequencing technique (RNA-seq). A total of 18,309 transcripts were detected in the current sequencing study, in which 593 transcripts were selected out according to the criteria: |log2(fold change)| > 1, P < 0.05 (22 up-regulated and 571 down-regulated). To explore whether the mRNA change induced by Neat1 knockdown in a stressed culture condition was different from that in normal condition, the cells were dealt with OGD and followed by RNA-seq. A total of 18,502 transcripts were detected, in which 121 transcripts were significantly up-regulated and 126 transcripts were significantly down-regulated according to the aforementioned filter rules.

2019-08-17 | GSE85749 | GEO

Chromatin profiling in HT22 hippocampal cells

Project description:We conducted genome-wide analysis of KMT2D binding sites in a hippocampal cell line to define KMT2D-dependent functions in neuronal context

2019-02-07 | GSE126166 | GEO

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