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Single cell immune response to the mRNA SARS-CoV-2 vaccine and yellow fever in the lymph nodes of mice

ABSTRACT: Single cell immune response to the mRNA SARS-CoV-2 vaccine and yellow fever in the lymph nodes of mice

PROVIDER: PRJNA742383 | ENA |

REPOSITORIES: ENA

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Single cell immune response to the mRNA SARS-CoV-2 vaccine and yellow fever in the lymph nodes of mice

Project description:Purpose: to understand the mechanisms of vaccines in the lymph nodes of mice Methods: mice were treated with mRNA SARS-CoV-2 vaccine or the yellow fever vaccine. The draining inguinal or illiac lymph nodes were harvested 1, 3, or 7 days after treatment and analyzed by scRNA-seq Results: TBD Conclusions: TBD

2022-01-30 | GSE179131 | GEO

Single Cell RNA-seq of the draining lymph nodes of mice treated with either 3M052 or Yellow Fever Vaccine (YF)

Project description:Purpose: To understand the innate immune response to an adjuvant, 3M052, and yellow fever vaccine, YFV Methods: Draining lymph nodes were negatively selected for CD19+ and CD3+, then flow sorted into four populations: Dendritic cells (DCs), Double positive cells (DP, CD11b+BST1+), Ly6c+ cells (Ly6c), and plasmacytoid dendritic cells (pDCs). Lymph nodes were harvested at baseline (D0), 24 hours post-treatment (D1) or 28 days post-treatment (D28). Results: TBD Conclusions: TBD

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Single Cell RNA-seq of the draining lymph nodes of mice treated with either 3M052 or Yellow Fever Vaccine (YF)

Project description:Single Cell RNA-seq of the draining lymph nodes of mice treated with either 3M052 or Yellow Fever Vaccine (YF)

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scRNAseq of stromal cells isolated from mesenteric lymph nodes (MLN), peripheral lymph nodes (PLN) and mandibular lymph nodes (mandLN)

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Immune response to the yellow fever vaccine 17D.

Project description:Correlates of immune mediated protection to most viral and cancer vaccines are still unknown. This impedes the development of novel vaccines to incurable diseases such as HIV and cancer. In this study, we have used functional genomics and polychromatic flow cytometry to define the signature of the immune response to the yellow fever (YF) vaccine 17D (YF17D) in a cohort of forty volunteers followed for up to one year after vaccination. We show that immunization with YF17D leads to an integrated immune response that includes several effector arms of innate immunity including complement, the inflammasome and interferons, as well as adaptive immunity as shown by an early T cell response followed by a brisk and variable B cell response. Development of these responses is preceded, as demonstrated in three independent vaccination trials and in a novel in vitro system of primary immune responses (Modular IMmune In vitro Construct (MIMIC) system), by the coordinated up-regulation of transcripts for specific transcription factors including STAT1, IRF7 and ETS2 that are upstream of the different effector arms of the immune response. These results clearly show that the immune response to a strong vaccine is preceded by coordinated induction of masters transcription factors, that lead to the development of a broad, polyfunctional and persistent immune response that integrates all effector cells of the immune system.

2008-12-01 | GSE13699 | GEO

Transcriptional profiling of YF-naïve participants after vaccination against yellow fever.

Project description:Mechanisms of poor responses to vaccines remain unknown. Yellow fever-naïve adults were vaccinated with a yellow fever vaccine (YF-17D, Stamaril). Transcriptomic profilling of blood collected pre-vaccination and post-vaccination (day 3, 7, 14 and 84) was performed in order to identify candidate biomarkers of antibody response to the vaccine.

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Molecular correlates of vaccine-induced protection against typhoid fever

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Immune response genes at the mRNA vaccine delivery sites and draining lymph nodes of rhesus macaques

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The BNT162b2 vaccine elicits robust lymph node T follicular helper cell responses

Project description:COVID-19 mRNA vaccines generate high concentrations of circulating anti-Spike antibodies and Spike-specific CD4+ T cells following prime-boost vaccination. It is not yet clear if vaccine-induced CD4+ T cell responses in the draining lymph node contribute to this outstanding immunogenicity. Using fine needle aspiration of draining axillary lymph nodes from individuals who received the BNT162b2 mRNA vaccine, we found large populations of Spike-specific CD4+ T follicular helper cells in the draining lymph node. A broadly immunodominant HLA-DPB1*04-restricted response to Spike166-180 composes one of the largest populations of T follicular helper cells in individuals with this allele, which is itself among the most common HLA alleles in the human population. Spike-specific T follicular helper cells are present in the lymph node 30 days after vaccine boost and persist in some individuals more than 170 days. Collectively, our results underscore the key role that robust T follicular helper cell responses play in the establishment of long-term immunity in this very efficacious human vaccine.

2022-01-04 | GSE183393 | GEO

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