Project description:Small RNAs were isolated from the hyperthermophilic archaeon Thermoproteus tenax and subjected to Illumina Hiseq2000 sequencing. The RNA reads revealed significant abundance of constitutively transcribed CRISPR RNAs and a plethora of C/D box sRNAs. The presence of a rare RNase P RNA variant was verified. Circular RNA molecules could be identified.
Project description:To investigate the gene expression of lung epithelial cells effected by Trichomonas tenax, we chose A549 lung epithelial cells and cocultured with Trichomonas tenax at MOI1.
Project description:To investigate the gene expression of lung epithelial cells effected by Trichomonas tenax, we chose NCI-H292 lung epithelial cells and cocultured with Trichomonas tenax.
Project description:In order to unravel the role of regulation on transcript level in the central carbohydrate metabolism (CCM) of Thermoproteus tenax (glycolytic/gluconeogenic carbon switch), the focused DNA microarray was constructed by using ORFs supposed to be involved in the CCM pathways. Transcriptional profiling was performed comparing autotrophic growth on CO2/H2 versus heterotrophic growth on glucose.
