Transcriptomic analysis of in vitro induced germinal centre-like B cells and plasmablast differentiation
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA743057 | ENA |
REPOSITORIES: ENA
Dataset's files
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| SRR15015600.fastq.gz | Fastqsanger.gz | |||
| SRR15015601.fastq.gz | Fastqsanger.gz | |||
| SRR15015602.fastq.gz | Fastqsanger.gz | |||
| SRR15015603.fastq.gz | Fastqsanger.gz | |||
| SRR15015598.fastq.gz | Fastqsanger.gz |
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Publications
A functional screen of RNA binding proteins identifies genes that promote or limit the accumulation of CD138+ plasma cells.
Turner David J DJ Saveliev Alexander A Salerno Fiamma F Matheson Louise S LS Screen Michael M Lawson Hannah H Wotherspoon David D Kranc Kamil R KR Turner Martin M
eLife 20220422
To identify roles of RNA binding proteins (RBPs) in the differentiation or survival of antibody secreting plasma cells we performed a CRISPR/Cas9 knockout screen of 1213 mouse RBPs for their ability to affect proliferation and/or survival, and the abundance of differentiated CD138 + cells in vitro. We validated the binding partners CSDE1 and STRAP as well as the m<sup>6</sup>A binding protein YTHDF2 as promoting the accumulation of CD138 + cells in vitro. We validated the EIF3 subunits EIF3K and ...[more]
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Project description:Currently very little is known about the differential expression and function of the transcription factor SOX5 during B cell maturation. We identified two new splice variants of SOX5 in human B cells, encoding the known L-SOX5B isoform and a new shorter isoform L-SOX5F. The SOX5 transcripts are highly expressed during late stages of B-cell differentiation, including atypical memory B cells, activated CD21low B cells and germinal center B cells of tonsils. In tonsillar sections SOX5 expression was predominantly polarized to centrocytes within the light zone. After in vitro stimulation, SOX5 expression was down-regulated during proliferation while high expression levels were permissible for plasmablast differentiation. Overexpression of L-SOX5F in human primary B lymphocytes resulted in reduced proliferation, less survival of CD138neg B cells, but comparable numbers of CD138+CD38hi plasmablasts compared to control cells. Thus, our findings describe for the first time a functional role of SOX5 during late B cell development reducing the proliferative capacity and thus potentially affecting the differentiation of B cells during the germinal center response.
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2022-04-24 | GSE189819 | GEO