Project description:Prostate cancer is one of the most common tumors of the male genitourinary system. Emerging research have confirmed that circRNAs play an important role in the occurrence and development of tumors. However, the correlation of circular RNA with PCa remains unclear. Here, the circRNA expression profiles in PCa and paracancerous tissues were established by high-throughput sequencing.
Project description:We demonstrate a new high-throughput model where rapidly proliferating and easily handled induced pluripotent stem cells enable generation of human prostate tissue in vivo and, for the first time, in vitro. Using a co-culture technique with urogenital sinus mesenchyme, we recapitulated the in situ prostate histology, including the stromal compartment and the full spectrum of epithelial differentiation.
Project description:This study aims to find the specific lncRNA molecule relating BMs from LADC in situ tumor to BM tumor tissue using gene chip technology and, combined with biological information database, through the competitive endogenous RNA (ceRNA) mechanism for microRNA (miRNA) related to specific lncRNA, to verify the possible target genes and therefore to explore the role and mechanism of specific lncRNA in BMs from LADC. By detecting the gene expression profiles of lncRNA and mRNA in cancer tissues of five cases of LADC and the paired paracancerous tissue samples, it was found that there were a large number of differentially expressed lncRNAs and mRNAs in LADC tissues and paracancerous tissues. By detecting the gene expression profiles of lncRNA and mRNA in three cases of BMs from LADC and matched paracancerous tissues, it was found that there were significant differences in the expression of lncRNAs and mRNAs between BM tumors of LADC and paracancerous tissues. These findings will provide a scientific basis for the early diagnosis and targeted therapy of BMs from LADC for application in further studies. Differential expression of lncRNAs and mRNAs in cancer tissues of five cases of LADC and the paired paracancerous tissue samples, it was found that there were a large number of differentially expressed lncRNAs and mRNAs in LADC tissues and paracancerous tissues.
Project description:To support our research of colon cancer in human genome, we conducted massively parallel pyrosequencing of mRNAs (RNA-seq) using normal,paracancerouse and cancerous human colon tissues. We obtained a total of 29.9M reads from normal,33.0M reads from paracancerous and 36.5M reads from cancerous.The RNA-seq data derived from the sample illustrated the differencially expreesion genes among normal,paracancerous and cancerous colon tissues of human. 3 samples examined: normal tissue, paracancerous tissue, cancerous tissue.
Project description:This study aims to find the specific lncRNA molecule relating BMs from LADC in situ tumor to BM tumor tissue using gene chip technology and, combined with biological information database, through the competitive endogenous RNA (ceRNA) mechanism for microRNA (miRNA) related to specific lncRNA, to verify the possible target genes and therefore to explore the role and mechanism of specific lncRNA in BMs from LADC. By detecting the gene expression profiles of lncRNA and mRNA in cancer tissues of five cases of LADC and the paired paracancerous tissue samples, it was found that there were a large number of differentially expressed lncRNAs and mRNAs in LADC tissues and paracancerous tissues. By detecting the gene expression profiles of lncRNA and mRNA in three cases of BMs from LADC and matched paracancerous tissues, it was found that there were significant differences in the expression of lncRNAs and mRNAs between BM tumors of LADC and paracancerous tissues. These findings will provide a scientific basis for the early diagnosis and targeted therapy of BMs from LADC for application in further studies. Differential expression of lncRNAs and mRNAs in cancer tissues of three cases of BMs from LADC and the paired paracancerous tissue samples, it was found that there were a large number of differentially expressed lncRNAs and mRNAs in BMs tissues and paracancerous tissues.
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using transcriptome profiling (RNA-seq) to evaluate the effects of lactoferrin deficiency on the global transcriptome of the lung microenvironment of the B16 metastasis mice model. Methods: 2×10^5 B16-F10 cells were injected into WT and lactoferrin kncoutout (Lf-/-) mice (each group has 10 eight-weeks-old male mice) through tail vein. At 3 weeks post injection,the paracancerous lung tissue were isolated (not including any metastatic colonies). RNAs were extracted by Trizol and sequenced by Solexa high-throughput sequencing service (Oebiotech, Shanghai, China). Data were extracted and normalized according to the manufacturer’s standard protocol.Each group has two mice paracancerous lung tissues be tested. Results: There are significant gene expression profile changes between WT and Lf-/- tumor-bearing mice. Conclusions: Our study describes the global transciptome changes of paracancerous lung tissues from the B16 metastasis mice model under the influence of lactoferrin kncokout.