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MRNA sequencing analysis on cardiofibroblasts exposed to reduced graphene oxide (rGO) Nanomaterials

ABSTRACT: mRNA sequencing analysis on cardiofibroblasts exposed to reduced graphene oxide (rGO) Nanomaterials

PROVIDER: PRJNA745453 | ENA |

REPOSITORIES: ENA

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Methylation arrays (MethylationEPIC) of human lung epithelial BEAS-2B cells exposed to reduced graphene oxide

Project description:Nanomaterials have lots of promising applications, and concern has risen about their impact to human health. Here, we have analyzed the genome-wide DNA methylation changes associated to the exposure to reduced graphene oxide (rGO) in human lung epithelial cells. Six conditions were assayed, with two technical replicates per condition (12 arrays in total): control, 1 and 10 µg/mL of rGO for 15 or 30 days of exposure.

2019-09-16 | E-MTAB-7719 | biostudies-arrayexpress

A transcriptomic overview of lung and liver changes one day after pulmonary exposure to graphene and graphene oxide

Project description:This study investigates transcriptomic differences in the lung and liver after pulmonary exposure to two Graphene based materials with similar physical properties, but different surface chemistry. Female C57BL/6 mouse were exposed by a single intratracheal instillation of 0, 18, 54 or 162 μg/mouse of graphene oxide (GO) or reduced graphene oxide (rGO). Pulmonary and hepatic transcriptional changes were compared to identify commonly and uniquely perturbed functions and pathways by GO and rGO. These changes were then related to previously analyzed endpoints. GO exposure induced more differentially expressed genes, affected more functions, and perturbed more pathways compared to rGO, both in the lung and liver.

2020-10-21 | GSE159707 | GEO

mRNA sequencing analysis on cardiofibroblasts exposed to reduced graphene oxide (rGO) Nanomaterials

Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to rGO on cardiofibroblasts (CFs) cells. We performed mRNA sequencing experiments using CFs cells exposed to rGO for 24h.

2021-07-14 | GSE179878 | GEO

mRNA microarray analysis on HepG2 cells exposed to Grpahene oxide (GO) and reduced graphene oxide (rGO) Nanomaterials

Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to GO and rGO on Human hepatoma (HepG2) cells. We performed whole genome DNA microarray experiments using HepG2 cells exposed to GO and rGO for 24h. We used whole genome microarrays to screen for global changed in HepG2 transcription profiles and with subsequent quantitative analysis conducted on selected genes. 24h GO and rGO exposed HepG2 cells were used for total RNA extraction and hybridization on Affymetrix microarrays.

2013-08-21 | E-GEOD-50039 | biostudies-arrayexpress

mRNA microarray analysis on HepG2 cells exposed to Grpahene oxide (GO) and reduced graphene oxide (rGO) Nanomaterials

2013-08-21 | GSE50039 | GEO

Single-cell mass cytometry reveals the impact of graphene nanomaterials with human primary immune cells

Project description:The genome-wide transcriptome analysis highlight the increased biocompatibility on immune cells of graphene functionalized with amino groups (NH2) compared with graphene oxide (GO); reducing the cell metabolism disfunction. Moreover, GONH2 was found to polarizes T-cell and monocyte activation toward a T helper-1/M1 immune response.

2017-08-13 | GSE99929 | GEO

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Project description:mRNA microarray analysis on HepG2 cells exposed to Grpahene oxide (GO) and reduced graphene oxide (rGO) Nanomaterials

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Carbon nanotubes, but not graphene oxide, trigger chemokine production in primary human macrophages via MyD88 dependent NF-kB Pathway

Project description:The growing medical application of engineered nanomaterials (ENMs) necessitates better understanding of their molecular toxicity mechanisms governing immune effects. In the present study, a comprehensive mechanistic in vitro assessment of two carbon-based ENMs, single-walled carbon nanotubes (SWCNTs) and graphene oxide (GO), was performed using primary human monocyte-derived macrophages (HMDM) as a model. HMDM were exposed to SWCNTs and GO at concentrations 10–100 µg/ml for 24 h. Toxicity, reactive oxygen species (ROS) production and uptake of ENM by cells was measured. The changes in gene expression profile of HMDM in response to SWCNTs and GO were studied and the key stress response pathways identified. The transcriptomics and pathway enrichment analysis findings were verified by multiplex immunoassay and functional assays.

2017-09-30 | GSE83516 | GEO

Identification of circular RNAs expression pattern in caprine fetal fibroblast cells exposed to a chronic noncytotoxic dose of graphene oxide-silver nanoparticle nanocomposites

Project description:circRNA sequencing for 6 samples.The increasing use of graphene oxide-silver nanoparticle nanocomposites (GO-AgNPs) in biomedical sciences and research also increases the chances of human and animal exposure to its chronic non-toxic doses. However, the available information is truly little for profound understanding of the effect of low-dose chronic exposure to GO-AgNPs on the epigenetic modification of the organism. In this research, we have investigated the impact of non-toxic doses of GO-AgNPs (0.5 µg/mL for 10 weeks, this dose does not interfere cell viability and ROS level) on the differential expression of circular RNAs (circRNAs) in caprine fetal fibroblast cells (CFFCs). In addition, using bioinformatics tools we have predicted the functions of those differentially expressed circRNAs on CFFCs. Furthermore, we have validated the expression of ten circRNAs using qRT-PCR to ensure the reliability of the sequencing data. The results showed that the differentially expressed circRNAs potentially regulates the GO-AgNPs induced epigenetic toxicity through a regulatory network consisted of circRNAs, miRNAs and mRNAs. Therefore, the inclusion of epigenetics approaches is essential to assess the biosafety level of GO-AgNPs nanomaterials.

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Project description:As antimicrobials, graphene materials (GMs) may have advantages over traditional antibiotics due to their physical mechanisms of action which ensure less chance of development of microbial resistance. However, the fundamental question as to whether the antibacterial mechanism of GMs originates from penetration by their sharp edges or wrapping of the cell membrane, or from a combination of these and other mechanisms, remains controversial. In this study, we show both experimentally and theoretically that GMs with high surface oxygen content (SOC) predominantly attach in parallel to the bacterial cell surface when in the suspension phase. The interaction mode shifts to perpendicular interaction when the SOC reaches a threshold of ~ 0.3 (the atomic percent of O in the total atoms). However, neither mode necessarily kills bacteria. Rather, bactericidal activity depends on the interaction of GMs with surrounding biomolecules. Proteomic study shows that protein corona forms on the surface graphene oxide in LB medium, thus explain the different antibacterial activity in different culture medium.

2023-05-10 | PXD027909 | Pride

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