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RNA-seq analysis of freshly isolated and ex-vivo cultured human cord blood CD34+ cells

ABSTRACT: RNA-seq analysis of freshly isolated and ex-vivo cultured human cord blood CD34+ cells

PROVIDER: PRJNA745928 | ENA |

REPOSITORIES: ENA

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RNA-seq analysis of freshly isolated and ex-vivo cultured human cord blood CD34+ cells

Project description:RNA-seq analysis was performed to compare differentially expressed genes in freshly isolated and ex-vivo cultured human cord blood CD34+ cells. Mitochondrion related genes are upregulated in CD34+ hematopoietic stem and progenitor cells upon ex vivo culture. In vivo transplantation experiments demonstrate that stemness of CD34+ cells is significantly decreased due to oxidative stress induced by ex vivo culture.

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