Project description:To understand the polycomb repressive complex 2 (PRC2) function, we genetically deleted EED, which inactivated PRC2, in adult intestine and colon using (VillinCreER-T2;EEDf/f).
Project description:Given that SATB2 is expressed in differentiated colonic cells as well as the stem cells, we evaluated whether SATB2 might directly regulate the identity of the differentiated cells
Project description:CRISPR knock out the Satb2 or Foxd2 gene from mouse colonic organoids to validate core human and mouse large intestine stem cell enrich TFs.
Project description:We use ChIP-seq to understand the genomic binding sites of SATB2 in colonic epithelium and to study the shifting of CDX2 and HNF4A's genomic binding sites after SATB2 loss in colonic epithelium.