Project description:The molecular regulation mechanisms involved in stress tolerance remain largely unknown. Drunken horsegrass (Achnatherum inebrians), an important perennial bunchgrass in China, forms a naturally occurring symbiosis with an asexual symbiotic fungus Neotyphodium gansuense.To gain insight into the molecular mechanisms involved in the low temperature resistance of E+ drunken horsegrass, Solexa deep-sequencing was used to identify candidate genes showing differential expression.
Project description:<p>Gene expression is a biological process regulated at different molecular levels, including chromatin accessibility, transcription, and RNA maturation and transport. In addition, these regulatory mechanisms have strong links with cellular metabolism. Here we present a multi-omics dataset that captures different aspects of this multi-layered process in yeast. We obtained RNA-seq, metabolomics, and H4K12Ac ChIP-seq data for wild-type and mip6delta strains during a heat-shock time course. Mip6 is an RNA-binding protein that contributes to RNA export during environmental stress and is informative of the contribution of post-transcriptional regulation to control cellular adaptations to environmental changes. The experiment was performed in quadruplicate, and the different omics measurements were obtained from the same biological samples, which facilitates the integration and analysis of data using covariance-based methods. We validate our dataset by showing that ChIP-seq, RNA-seq and metabolomics signals recapitulate existing knowledge about the response of ribosomal genes and the contribution of trehalose metabolism to heat stress.</p>
