Project description:We injected mature (4-months-old) workers and gamergates with siRNAs against Kr-h1 or, as a control, GFP and performed RNA-seq on their ovaries 24 h later
Project description:We performed ChIP-seq with a new Kr-h1 antibody in the central brains of in established gamergates (day 120) and age-matched workers
Project description:Transcriptome analysis (RNA-Seq) was performed to identify Kr-h1 target genes. Message RNAs were extracted from wildtype and kr-h1[7] homozygous 3rd instar larvae at 100 hr after egg laying (two biological replicates for each genotype).
Project description:In ant Harpegnathos saltator, workers can become the reproductive gamergates in a queenless condition. During the worker-to-gamergate transition, juvenile hormone signaling decreases but ovarian ecdysone production increases in the gamergate. Injecting juvenile hormone analog (JHA or methoprene) induces expression of worker-biased genes in the brain , while ecydsone promotes expression of ecdysone-responsive genes in ovaries.
Project description:Harpegnathos workers were induced to transition to reproductive gamergates and 120 days later mRNA from their brains was sequenced to determine changes in gene expressioin