Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of Transcription Profiles of M. smegmatis overexpressing wild type and phopshorylation defective PknK

ABSTRACT: Comparison of Transcription Profiles of M. smegmatis overexpressing wild type and phopshorylation defective PknK

PROVIDER: PRJNA747928 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Comparison of Transcription Profiles of M. smegmatis overexpressing wild type and phopshorylation defective PknK

Project description:We have shown that elevated levels of wild-type M. tuberculosis PknK (PknK Mtb), but not phosphorylation-defective PknKMtb, in Mycobacterium smegmatis cause significant retardation of growth rate and altered colony morphology (Malhotra et al., 2012). LIX79 (WT PknK) and LIX80 (PknK K55M mutant) M. smegmatis strains will be grown in LB and induced with 0.2% acetamide for the induction of wild type or phosphorylation defective pknK gene. RNA and Protein will be isolated from cultures pelleted at times—24 h (24h) and 96 h (96 h) post induction. M. smegmatis strain LIX70 containing the empty vector will be induced at the same time-points and used as a control in hybridization. Two independent replicate experiments will be done.

2022-03-10 | GSE180348 | GEO

Mycobacterium smegmatis - Comparison of wild type SMR5 and glnR deletion strain MH1 under nitrogen starvation

Project description:In this approach, total transcript of the Mycobacterium smegmatis wild type SMR5 and a glnR deletion strain was compared under nitrogen starvation. Transcript levels of 6 genes were enhanced, 125 genes reduced in the mutant strain. This experiment confirmed GlnR as the global regulator of nitrogen metabolism in M. smegmatis. Two biological replicates were analyzed.

2011-07-01 | GSE30234 | GEO

Mycobacterium smegmatis - Comparison of amtR deletion strain YL1 under nitrogen surplus and starvation

Project description:In this approach, total transcript of a Mycobacterium smegmatis amtR deletion strain was compared under nitrogen surplus and starvation. This is a control experiment to the comparison of wild type and amtR deletion strain, resulting nitrogen-related genes which are not AmtR-controlled. Two biological replicates were analyzed.

2011-07-01 | GSE30232 | GEO

Mycobacterium smegmatis - Comparison of glnR deletion strain MH1 under nitrogen surplus and starvation

Project description:In this approach, changes in total transcript of a glnR deletion strain of Mycobacterium smegmatis due to nitrogen starvation were monitored. This is a control experiment for the comparison of wild type and glnR deletion strain resulting in putative nitrogen-related genes which are not controlled by GlnR.

2011-07-01 | GSE30033 | GEO

Mycobacterium smegmatis - Comparison of wild type SMR5 under nitrogen surplus and starvation

Project description:In this approach, total transcript of the Mycobacterium smegmatis wild type SMR5 was compared under nitrogen surplus and starvation. This resulted in the change of transcript patterns of more than 500 genes due to nitrogen or general starvation response. Genes involved in uptake and assimilation of ammonium and alternative nitrogen sources showed enhanced transcript levels, genes involved in cell growth, protein biosynthesis etc showed reduced transcript levels. Two biological replicates were analyzed.

2011-07-01 | GSE30233 | GEO

Mycobacterium smegmatis - Comparison of wild type SMR5 and glnR deletion strain MH1 under nitrogen starvation

2011-07-01 | E-GEOD-30234 | biostudies-arrayexpress

Mycobacterium smegmatis - Comparison of wild type SMR5 and amtR deletion strain YL1 under nitrogen surplus

Project description:In this approach, total transcript of the Mycobacterium smegmatis wild type was compared to a amtR deletion strain under nitrogen surplus. With this experiment, putative target genes of AmtR, a regulator protein of nitrogen metabolism, were identified. Two biological replicates were analyzed.

2011-07-01 | GSE30235 | GEO

Mycobacterium smegmatis - Comparison of wild type SMR5 and amtR deletion strain YL1 under nitrogen starvation

Project description:In this approach, total transcript of the Mycobacterium smegmatis wild type was compared to a amtR deletion strain under nitrogen starvation. With this experiment, putative target genes of AmtR, a regulator protein of nitrogen metabolism, were identified. Two biological replicates were analyzed.

2011-07-01 | GSE30231 | GEO

RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r)

Project description:RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) to assess the impact of Butters lysogen and specifically Buttersgp57r on transcript levels of island3 during infection.

2022-12-24 | GSE221406 | GEO

Comparison of wild-type Synechocystis sp. PCC 6803 with strains overexpressing wild-type or 5’-sensing-deficient RNase E

Project description:We compared transcriptomic changes, 5'-triphosphorylated (TSS) and 5'-monophosphorylated (PSS) RNA ends of different strains of the cyanobacterium Synechocystis sp. PCC6803. Comparison encompassed wild-type Synechocystis (WT), a strain overexpressing RNase E and RNase HII (rne(WT)) and a strain overexpressing 5’-sensing-deficient RNase E and RNase HII (rne(5p)). Analysis of changing 5'-monophosphorylated ends revealed 5’ sensing depedent processing sites on a transcriptome-wide level.

2023-01-20 | GSE184824 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data