Project description:We investigated the epigenetic plasticity of adult (postnatal day (P) 28) murine Müller glia using whole-genome bisulfite sequencing (WGBS)
Project description:Upon toll-like receptor (TLR) ligation, dendritic cells (DCs) undergo an activation process referred to as maturation. Over the course of maturation, global DC gene expression is severely altered, resulting in phenotypic changes including DC morphology, migration, immune stimulatory and inhibitory receptor expression, cytokine production, etc. We performed microarray analysis to investigate changes in gene expression patterns between wild type and MK2-deleted murine splenic DCs over time (0 - 24 hours) following TLR stimulation by LPS.
Project description:We used whole genome bisulfite sequencing (WGBS) to determine the DNA methylation levels at single base-pair resolution of four patients with different subtypes of pediatric ALL together with RNA-sequencing to increase our understanding of the leukemic transformation in ALL.
Project description:Whole genome bisulfite sequencing (WGBS) of the NA18507 (Yoruba) lymphoblastoid cell line high resolution methylome of one cell type
Project description:Diet plays an important role in lifestyle disorders and our study from Methionine Choline deficient diet induced non-alcoholic fatty liver disease led to specific decrease in pDC fraction from murine spleens. Delineating the role for individual components identified correlation of L-methionine supplementation with representation of pDC subtype. Considering implication of L-methionine in maintaining methylation status, we investigated the DNA methylation profiles among DC subtypes. Whole genome bisulfite sequencing analysis identified that pDCs display distinctly methylated regions in comparison to cDC subsets whereas comparable methylation in cDC1 and cDC2 served as a control. Further analysis of the pathways associated with DMRs, we reported that inhibition of MAPK pathway guides DC development towards pDC subtype. We demonstrate that supplementation with S-adenosylmethionine but not Homocysteine reversed the defect in pDC development induced by methionine deficiency. Overall, our study identifies important role for methionine in pDC biology.
