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Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to ACTH and Angiotensin II

ABSTRACT: Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to ACTH and Angiotensin II

PROVIDER: PRJNA750812 | ENA |

REPOSITORIES: ENA

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Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to ACTH and Angiotensin II

Project description:RNA sequencing provides a transcriptome-wide view of what processes are activated and repressed during steroidogenesis. Here we performed an RNA-seq time series on primary human adrenocortical cells and H295R cells stimulated with ACTH or AngII (forskolin for H295R). We found that the ligand-induced changes in gene expression largely involved the same genes and similar timing. While stimulus-induced expression changes in H295R cells are recapitulated by primary cells, there are also expression changes unique to primary cells.

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Angiotensin II early regulated genes in H295R human adrenocortical cells

Project description:H295R human adrenocortical cells treated with or without angiotensin II (100 nM) for 3 hr. Keywords = adrenal angiotensin aldosterone Keywords: parallel sample

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H295R human adrenocortical cells treated with Angiotensin II, Potassium or Forskolin

Project description:H295R human adrenocortical cells were cultured in H295R complete media containing DMEM:F12 (1:1) supplemented with 2% Ultroser G (Biosepra, Villeneuve-la-Garenne, France), ITS-Plus (Discovery Labware, Bedford, MA) and an antibiotic/antimycotic mixture (Invitrogen, Carlsbad, CA) in 175 cm2 flasks until subconfluente. Media was replaced with 40 ml fresh media containing different agents and cultured for 3 h. Treatments: Angiotensin II (100 nM), potassium (16 mM) and forskolin (10 uM). Total RNA extracted with RNAesy Midi Kit (Qiagen) with on-column DNase digestion Keywords: Agent response

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Transcription profiling of human, Bos taurus, and rat adrenocortical cells treated with Angiotensin II

Project description:Angiotensin II (Ang-II) regulates adrenal steroid production and gene transcription through several signaling pathways. Changes in gene transcription occur within minutes after Ang-II stimulation, causing an acute increase in aldosterone production and subsequent increase in the overall capacity to produce aldosterone. Our goal was to compare the Ang-II regulation of early gene expression and confirm the upregulation of selected genes using quantitative real-time RT-PCR (qPCR) across three species: human, bovine, and rat. Experiment Overall Design: Microarray analysis was performed using samples from control and Ang-II-(10 nM) treated (1 hour) cells from human adrenocortical tumor cell line H295-R, and primary adrenal glomerulosa cells from bovine and rat, applied respectively to human HG-133 + 2 , bovine, and rat 230-2 Affymetrix chips. qPCR was performed to confirm upregulation of selected genes using mRNA. Dye Swap was not used. Human samples (H295R) include 3 replicates of Basal (controls) and 3 repeats of Angiotensin II samples. Human H295R cells include also a group of cycloheximide (protein synthesis blocker) and angiontensin II + cycloheximide in order to check if the genes were direct targets of angiotensin II.

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Genome-wide analysis of fetal adrenocortical cells gene expression after 48 h ACTH treatment

Project description:Analysis of ACTH-regulation on adrenocortical cells at gene expression level. The hypothesis tested in the present study was that ACTH increases chronic cell growth and steroidogenesis in adrenal glands by changing the gene expression profile. Results provide important information on the changes of gene expression of adrenocortical cells after chronic ACTH treatment.

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Genome-wide analysis of adult adrenocortical cells gene expression after 48 h ACTH treatment

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Genome-wide analysis of adult adrenocortical cells gene expression after 48 h ACTH treatment

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Genome-wide analysis of fetal adrenocortical cells gene expression after 48 h ACTH treatment

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Project description:Profiling of Angiotensin II Rapid Response Genes in Human, Bovine, and Rat Adrenocortical Cells.

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Profiling of Angiotensin II Rapid Response Genes in Human, Bovine, and Rat Adrenocortical Cells.

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