Project description:We identified 2 leading gene candidates (G0s2 and Rgs16) in response to circadian, diet and genetic manipulations by a large-scale transcriptome analysis. To examine the impact of G0s2 and Rgs16 on liver functions, we carried out adenovirus-mediated liver-specific knockdown of both genes and performed RNA-seq analysis.
Project description:Cytochrome P450 oxidoreductase (POR) is involved in the oxidative metabolism of xenobiotics and endogenous compounds like fatty acid and cholesterol. While effects of diminished Por were extensively studied in mouse models, studies in human models are still missing. We used previously established CRISPR/Cas9-mediated POR knockdown in HepaRG cells as a human hepatic cell model to investigate the effects of POR knockdown on global protein expression levels.
Project description:To gain insight into the signaling pathway(s) required for ABL1/ABL2-dependent bone metastasis, we evaluated the consequences of single or double inactivation of ABL1 and ABL2 on the transcriptome of breast cancer cells. Double ABL1/ABL2 knockdown was required to decrease the levels of p-CrKL by more than 90%, indicative of inactivation of the endogenous ABL kinases. To examine the consequences of depleting the ABL kinases on the transcriptome of metastatic breast cancer cells we employed next generation sequencing (RNAseq) analysis. We found that 180 genes were significantly down-regulated and 40 genes were significantly up-regulated in ABL1/ABL2 knockdown cells. Four samples were analyzed control, Abl single knockdown, Arg single knockdown, Abl/Arg double knockdown. Experiments were performed in triplicate.
Project description:To investigate the effect of Cell-Adhesive Nanofibril on the hepatic differentiation process of 3D iPSC spheroids, we established hepatic organoids that underwent a stepwise hepatic differentiation process with or without Cell-Adhesive Nanofibril.
Project description:To test the effect of Spag4 knockdown, we compared knockdown and control cells subjected to 48h 2uM camptothecin treatment or vehicle.