Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sustained experimental activation of FGF8/ERK in the developing chicken spinal cord reproducibly models early events in ERK-mediated tumorigenesis

ABSTRACT: Sustained experimental activation of FGF8/ERK in the developing chicken spinal cord reproducibly models early events in ERK-mediated tumorigenesis

PROVIDER: PRJNA754401 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Sustained experimental activation of FGF8/ERK in the developing chicken spinal cord reproducibly models early events in ERK-mediated tumorigenesis

Project description:Most human cancers demonstrate activated MAPK/ERK pathway signaling as a key tumor initiation step, but the immediate steps of further oncogenic progression are poorly understood due to a lack of appropriate models. Spinal cord differentiation follows caudal elongation in vertebrate embryos; both processes are regulated by a FGF8 gradient highest in neuromesodermal progenitors (NMP), where kinase effectors ERK1/2 maintain an undifferentiated state. FGF8/ERK signal attenuation is necessary for NMPs to progress to differentiation. We show that sustained ERK1/2 activity, using a constitutively active form of the kinase MEK1 (MEK1ca) in the chicken embryo, reproducibly provokes neopasia in the developing spinal cord. Transcriptomic data show that neoplasia not only relies on the maintenance of NMP gene expression, and the inhibition of genes expressed in the differentiating spinal cord, but also on a profound change in the transcriptional signature of the spinal cord cells leading to a complete loss of cell-type identity. MEK1ca expression in the developing spinal cord of the chicken embryo is therefore a tractable in vivo model to identify the critical factors fostering malignancy in ERK-induced tumorigenesis.

2021-08-17 | GSE182072 | GEO

Sustained experimental activation of FGF8/ERK in the developing chicken spinal cord models early events in ERK-mediated tumorigenesis.

Project description: Not available

| S-EPMC8717438 | biostudies-literature

Proteosome-Erk dependent transcriptional events

Project description:Transcriptome analysis of ESCs treated with a proteosome inhibitor (MG132 1μM), with our without ERK activity to measure Erk-dependent changes in transcriptome regulation

2019-08-08 | GSE110877 | GEO

Proteosome-Erk dependent transcriptional events

Project description:Proteosome-Erk dependent transcriptional events

| PRJNA434732 | ENA

Global expression analysis identified a preferentially NGF-induced transcriptional program regulated by sustained MEK/ERK and AP-1 activation during PC12 differentiation.

2011-11-13 | GSE33639 | GEO

Global expression analysis identified a preferentially NGF-induced transcriptional program regulated by sustained MEK/ERK and AP-1 activation during PC12 differentiation.

Project description:Neuronal differentiation of PC12 cells in response to NGF is a prototypical model in which signal duration determines a biological response. Sustained ERK activity induced by NGF, as compared to transient activity induced by EGF, is critical to the differentiation of these cells. To characterize the transcriptional program activated preferentially by NGF, we compared global gene expression profiles between cells treated with NGF and EGF for 2-4 hrs, when sustained ERK signaling in response to NGF is most distinct from the transient signal elicited by EGF. This analysis identified 69 genes that were preferentially upregulated in response to NGF. PC12 cells that were starved in low serum media for 24 hrs were treated with NGF (50ng/mL) or EGF (25ng/mL) for 2 or 4 hrs, or left untreated. Total RNA for 3 independent biological replicates was extracted and subjected to Affymetrix Rat Gene 1.0ST Arrays. The 69 genes that were preferentially upregulated by NGF compared to EGF met the following criteria: NGF/No treatment log2> 1, FDR p value< 0.01 and NGF/EGF log2> 0.75, FDR p value< 0.01.

2011-11-13 | E-GEOD-33639 | biostudies-arrayexpress

Effect of FGF8 on bovine granulosa cells

Project description:FGF8 induction of immediate-early response genes

2013-10-10 | GSE41489 | GEO

Effect of FGF8 on bovine granulosa cells

Project description:FGF8 induction of immediate-early response genes Cells treated for 2 h vs untreated controls. Three biological replicates

2013-10-10 | E-GEOD-41489 | biostudies-arrayexpress

Fgf8 E18.5 hypomorph versus control mouse fetal lungs

Project description:We set out to look for genome wide gene expression changes due to deficiency of Fgf8 in the lung.

2010-09-20 | GSE22893 | GEO

Transcription profiling of animal caps from Danio rerio embryos expressing GFP or vhnf1 and treated with FGF8 or BSA protein

Project description:Zebrafish embryo animal cap explants were differentiated by injection of vhnf1 mRNA into the one-cell stage embryo, and treatment of excised explants with applied FGF8 protein. To identify unique targets of the combination of vhnf1+FGF8, each factor was applied alone and in combination, and differences in gene expression used to identify unique targets of vhnf1+FGF8.

2007-05-01 | E-MEXP-818 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data