Project description:In contrast to its common definition and calculating process, interpretations of p-value differ among statisticians. Since p-value is the basis of various methodologies, this divergence has led to distinct test methodologies as well as various opinions for evaluating test results, producing a chaotic situation. Here the origin of the divergence is found in the differences among Pr(H0 = true), which gives a prior probability in the definition of p-values. Effects of differences in the prior probability on the character of p-values are investigated by comparing microarray data and random numbers as subjects. The summarized levels the genes are presented in the matrix files (linked below as supplementary files). Student t-test was applied between the two groups (0h and 14d): p-values presented in the matrix files.
Project description:Test data used for the evaluation of ESAT performance and results files for data from 3' and 5' end-sequencing RNA-Seq protocols and droplet-based single-cell RNA-Seq.
Project description:Neurog3 and NeuroD1 share the ability to induce endocrine differentiation when expressed ectopically in vivo (Apelqvist et al., 1999; Schwitzgebel et al., 2000; Grapin-Botton et al., 2001) and in vitro (Heremans et al., 2002; Gasa et al., 2004), suggesting that they target a common set of genes. To test this hypothesis, we directly compared in the same cells the effects of Neurog3 and NeuroD1 on global gene expression patterns. Normalized M and A values [limmaGUI output] for the neurogenin3 (Ngn3) and neuroD1 (Nd1) experiments (individual or combined) have been linked below as Supplementary files. Note that M=log2(test/ref), eg, log2(Nd1/Bgal); A=[log2(test*ref)]/2, eg, [log2(Nd1*Bgal)]/2. Additional measures of reliability/probability are also included within the supplementary files. Keywords: comparative genomic hybridization, neurogenin3, neuroD, ductal cells, endocrine, differentiation
