Project description:Citrus Huanglongbing (HLB) is the most devastating citrus disease in the world. Candidatus Liberibacter asiaticus (Las) is the prevalent HLB pathogen, which is yet to be cultivated. A recent study demonstrates that Las does not contain pathogenicity factors that are directly responsible for HLB symptoms. Instead, Las triggers systemic and chronic immune responses. Importantly, overproduction of reactive oxygen species (ROS) causes systemic cell death of phloem tissues, thus causing HLB symptoms. Because Las resides in the phloem tissues, it is expected that phloem cell might recognize outer membrane proteins, outer membrane vesicle (OMV) proteins and extracellular proteins of Las to contribute to the immune responses. Because Las has not been cultivated, we used Liberibacter crescens (Lcr) as a surrogate to identify the OMPs, OMV proteins and extracellular proteins by liquid chromatography with tandem mass spectrometry (LC-MS/MS). We observed OMVs of Lcr under scanning electron microscope, representing the first experimental evidence that Liberibacter can deliver proteins into the host. In addition, we also further analyzed LC-MS/MS data using bioinformatic tools. Our study provides valuable information regarding the biology of Ca. Liberibacter species and identifies many putative proteins that may interact with host proteins in the phloem tissues.

2022-07-27 | MSV000089995 | MassIVE

Emmonsia crescens

Project description:Emmonsia crescens overview

| PRJNA178779 | ENA

Complete genome sequence of Liberibacter crescens BT-1.

Project description: Not available

| S-EPMC3569387 | biostudies-literature

Emmonsia crescens strain:UAMH4076

Project description:Emmonsia crescens UAMH4076 Genome sequencing

| PRJNA252751 | ENA

Barley low temperature stress

Project description:In this study we used the Affymetrix Barley 1 GeneChip to investigate transcriptome responses of barley cv. Morex to low temperature, including triplicated measurements of cold, freeze/thaw cycles and de-acclimation over 33 days. Keywords: stress response

2008-02-02 | GSE6993 | GEO

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