Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

V. cholerae transcriptome without treatment and with indole or sub-MIC tobramycin

ABSTRACT: V. cholerae transcriptome without treatment and with indole or sub-MIC tobramycin

PROVIDER: PRJNA756892 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

V. cholerae transcriptome without treatment and with indole or sub-MIC tobramycin

Project description:We study gene expression levels of V. cholerae N16961 hapR+ corrected strain in exponential phase without and with indole or tobramycin.

2021-08-26 | GSE182561 | GEO

Effect of sub-MIC tobramycin treatment on E. coli MG1655 WT and NCM3416

Project description:Effect of sub-MIC tobramycin treatment on E. coli MG1655 WT (strain classically used as WT strain) and NCM3416 (corrected rph strain). Comparison of genes expression without treatment (MH) in E. coli MG1655 WT and NCM3416.

2023-03-01 | GSE224240 | GEO

Kristen-PAO1 aerobic sub-inhibitory cond. tobramycin

Project description:To see the effect of sub-inhibitory concentrations of tobramycin on Pseudomonas aeruginosa grown under aerobic conditions. RNA was isolated form 4 biological repeats of P.aeruginosa grown to mid-log in cationic adjusted mueller hinton broth and 4 biological repeats of P.aeruginosa with the addition of 0.25ug/ml tobramycin in cationic adjusted mueller hinton broth. null

2011-03-30 | E-FPMI-23 | biostudies-arrayexpress

Kristen-PAO1-anaerobic sub-lethal concentrations tobramycin

Project description:To see the effect of sub-lethal concentration of tobramcyin on PAO1 under anaerobic conditions. RNA was isolated from 5 biological repeats of PAO1 grown to mid-log phase in cationic adjusted mueller hinton broth containing 15mM KNO3 and 5 biological repeats of PAO1 grown to mid-log phase in cationic adjusted mueller hinton broth containing 15mM KNO3 and then treated for 30 minutes with 2 ug/ml tobramycin. All flasks were sealed to create anaerobic conditions. null

2011-01-20 | E-FPMI-25 | biostudies-arrayexpress

V. cholerae transcriptome or deleted for the tgt gene in MH, supplemented or not with tobramycin or ciprofloxacin

Project description:We study gene expression levels of V. cholerae N16961 WT and tgt mutant strains in MH medium in the presence or not of sublethal concentration on tobramycin or ciprofloxacin

2022-10-03 | GSE214520 | GEO

Transposon Insertion Sequencing to study the genetic interactions between V. cholerae WT cells and two mutant strains in absence or presence of subinhibitory doses of tobramycin

Project description:This study was conducted to study which genes or functions become important or expendable in two V. cholerae mutants compared to the WT. This was assessed directly by conjugating a Mariner Transposon with WT or mutant cells and obtaining a bank of hundreds of thousands of mutants which we consider as the T=0. We then subjected these banks to 16 generations in rich media with or without 50% MIC of tobramycin (which we call T=16gen). At both T0 and T16gen we extracted the genomic DNA of these banks and sequenced it to identify the regions where the transposon is inserted in each strain at the different conditions.

2023-04-26 | GSE198341 | GEO

Tobramycin Treatment of Planktonic Pseudomonas aeruginosa

Project description:As a comparison to tobramycin-treated P. aeruginosa biofilms, we investigated the response of planktonic P. aeruginosa to tobramycin by microarray. Keywords: Tobramycin Response

2008-06-10 | GSE9991 | GEO

Expression data from Pseudomonas aeruginosa PAO1 and its isogenic ampR mutant in the presence and absence of sub-MIC ß-lactam exposure.

Project description:The transcriptional regulator AmpR controls expression of the AmpC ß-lactamase in P. aeruginosa and other bacteria. Studies have demonstrated that in addition to regulating ampC expression, AmpR also regulates the expression of the sigma factor AlgT/U and the production of some quorum-sensing regulated virulence factors. In order to understand the ampR regulon, we compared the expression profiles of PAO1 and its isogenic ampR mutant, PAO∆ampR in the presence and absence of sub-MIC ß-lactam stress. The analysis demonstrates that the ampR regulon is much more extensive than previously thought, with the deletion of ampR affecting the expression of over 300 genes. Expression of an additional 207 genes are affected by AmpR when the cells are exposed to sub-MIC ß-lactam stress, indicating that the ampR regulon in P. aeruginosa is much more extensive than previously thought. An inframe deletion of ampR was generated in P. aeruginosa PAO1. The wild type and ampR mutant strains were grown to mid-log phase and subjected to sub-MIC ß-lactam exposure. Total RNA was isolated from 2-hour ß-lactam exposed and unexposed cells to monitor changes in gene expression arising due to loss of ampR in the presence and absence of ß-lactam exposure.

2012-03-29 | E-GEOD-33188 | biostudies-arrayexpress

Expression data from Pseudomonas aeruginosa PAO1 and its isogenic ampR mutant in the presence and absence of sub-MIC ß-lactam exposure.

2012-03-30 | GSE33188 | GEO