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ATAC-seq on DHT treated/untreated prostate cell line RWPE1

ABSTRACT: ATAC-seq on DHT treated/untreated prostate cell line RWPE1

PROVIDER: PRJNA760301 | ENA |

REPOSITORIES: ENA

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ATAC-seq on DHT treated/untreated prostate cell line RWPE1

Project description:Precise gene regulation is needed for the correct differentiation and function of cells. This is especially important when putting cells through stimuli that would change their characteristics. To understand the changes in chromatin accessibility of prostate cells when stimulating them with testosterone, we performed the ATAC-seq (Assay for Transposase Accessible Chromatin using sequencing). We have worked with the normal epithelial prostate cell line RWPE1, an heterogenous cell line composed of two subpopulations: WPE-stem (stem cell phenotype) and WPE-int (intermediate cellular subtype) and treated it with DHT. Our aim was to study haemoglobin gene regulation in prostate cells, and check whether changes in cell stimulus would lead to changes in chromatin accessibility in both haemoglobin loci. Nevertheless, the results obtained could be used to compare chromatin accessibility between DHT treated and untreated cells on any loci.

2021-09-06 | GSE183330 | GEO

RNA sequencing for RWPE1 cells, OLFM4-Active RWPE1 cells and OLFM4-KO RWPE1 cells

Project description:OLFM4 gene expressing in RWPE1 stem/progenitor-like cells. To study OLFM4 gene functions and molecular mechanisms, we generated OLFM4-avtive and OLFM4-knock out-GFP reporter RWPE1 cells with CRISP-Cas 9 system. The cell transcriptome was studied with RNA sequencing.

2019-08-05 | GSE126162 | GEO

RNA sequencing for RWPE1 cells, OLFM4-Active RWPE1 cells and OLFM4-KO RWPE1 cells

Project description:RNA sequencing for RWPE1 cells, OLFM4-Active RWPE1 cells and OLFM4-KO RWPE1 cells

| PRJNA521171 | ENA

Transcription profiling of human androgen receptor expressing prostate carcinoma cell line LNCaP and normal human foreskin fibroblasts expressing the androgen receptor treated with dihydrotestosterone (DHT), ethanol, or untreated vs. a common reference, see E-SMDB-3637

Project description:LNCaP cells are an established androgen receptor expressing prostate carcinoma cell line. Human foreskin fibroblasts also expressing the androgen receptor were obtained from phenotypic normal male individuals. Cells were cultured either at confluency leading to G0 cell cycle state or while they were proliferating. Cells were either untreated, or treated with dihydrotestosterone (DHT) or ethanol (ETOH) which also served as the solvent for the DHT. All experimental RNA samples derived from the untreated or treated cell lines were hybridized on cDNA arrays against a common reference. This reference was composed out of common reference CRG (50%) and out of fibroblast RNA (50%). This reference is also called "mixed reference" in the description of the 26 individual experiments.

2006-11-06 | E-SMDB-3636 | biostudies-arrayexpress

Myc and PI3-k induced gene expression in RWPE1

Project description:Analysis of transcriptional changes during Myc or PI3K induced oncogenic transformation in RWPE1 cells (benign prostate epithelial cell line). The aim of the present study was to identify key epigenetic gene silencing events that occur during the oncogenic transformation events, hence emphasis was placed on downregulated genes. Results provide important information on which are the tumor suppressive pathways or genes that will be epigenetically silenced by during Myc or PI3K induced oncogenic transformation. Total RNA obtained from oncogenic transformed RWPE1 cells which were either overexpressing Myc or constitutive active mutant of PI3K (E545K) as compared to RWPE1 cells expressing the empty vector control PMN.

2015-05-20 | E-GEOD-43686 | biostudies-arrayexpress

ChIP-seq from RWPE1 (ENCSR248IFK)

Project description:ChIP-seq on human RWPE1 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-10-20 | GSE105387 | GEO

ChIA-PET from RWPE1 (ENCSR030KAB)

Project description:CTCF ChIA-PET in RWPE1 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2021-06-15 | GSE176698 | GEO

ChIP-seq from RWPE1 (ENCSR203KEU)

Project description:H3K27ac ChIP-seq on human RWPE1 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-10-20 | GSE105290 | GEO

ChIA-PET from RWPE1 (ENCSR617FPZ)

Project description:RNAPII ChIA-PET with RWPE1 cell For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2021-11-04 | GSE187670 | GEO

ChIP-seq from RWPE1 (ENCSR303GFI)

Project description:CTCF ChIP-seq on human RWPE1 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-10-23 | GSE105468 | GEO

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