Project description:Comparing the transcriptomes of pairing-experienced (EM) and pairing-unexperienced (UM) male Schistosoma mansoni (in biological triplicates) we detected 253 genes to be significantly differeantially (p < 1-10; log2ratios < -0.585 or > +0.585) transcribed.
Project description:Comparing the transcriptomes of pairing-experienced (EM) and pairing-unexperienced (UM) male Schistosoma mansoni (in biological triplicates) we detected 253 genes to be significantly differeantially (p < 1-10; log2ratios < -0.585 or > +0.585) transcribed. pairing-experienced (EM) and pairing-unexperienced (UM) male Schistosoma mansoni were compared by SuperSAGE (Matsumura et al., 2003; with minor changes: Molina et al., 2008), sequencing Illumina (Solexa)
Project description:Transcriptional profiling using two subsequent developmental stages of Schistosoma mansoni (Egg vs. Miracidium; Cercaria vs. 7-days-old Schistosomulum; 7-days-old Schistosomulum vs. Adult worms
Project description:Schistosomiasis affects over 250 million people worldwide and is caused by trematodes of the genus Schistosoma including the species Schistosoma mansoni. Praziquantel (PZQ) is the most widely available and implemented drug for this disease, and is produced as an racematic mixture composed of the enantiomers, R-PZQ and S-PZQ. In this study we examined the gene expression profiles of 49 day male S. mansoni after 18 hour treatment with R-PZQ, S-PZQ or control (1% DMSO). These results provide insight into the effect of PZQ enantiomers against male S. mansoni.
Project description:Schistosoma mansoni is a dioecious species, that is, it has two differentiated sexes. Interestingly, this sexual species evolved from a hermaphrodite ancestor. Indeed, most Platyhelminthes are hermaphrodites. Here we characterize the microRNAs of S. mansoni and quantify their differential expression between males and females.
