Project description:We report the single nucleus multiome (RNAseq+ATACseq) of a male mouse pituitary sample. This dataset was generated for supporting the development of a novel computational framework for analyzing gene regulatorty circuitry from single cell multiome datasets. This computational framework extract regulatory circuits consisting of TFs, cis-regulatory sites and target genes by modelling the co-incidence of the RNA levels of the TFs, the chomatin accessibility of the TFs' binding sites and the RNA levels of target genes across single cell. Specifically we identified gonadotrope-specific regulatory circuits under the transcription factor Gata2. These regulatory circuits were validated by male mouse pituitary samples with gonadotrope-conditional knockout of Gata2 previously reported in GSE190066.
Project description:Identifying BTLA interacting proteins in mouse CD4+ effector T cells expressing BTLA at endogenous levels and after stimulation with pervanadate.
Project description:Identifying Smad4-binding proteins in mouse CD4+ T cells under IL6SB condition. Mouse CD4+ T cells were isolated from mouse spleen and lymph nodes.