Project description:We demonstrate induction and long-term maintenance of totipotent stem cells (TotiSCs) from mouse pluripotent stem cells (PSCs) by a combination of three small molecules, TTNPB, 1-Azakenpaullone, and WS6. These cells, which we designated as ciTotiSCs (chemically induced totipotent stem cells), resembled mouse totipotent 2C-embryo stage cells at both transcriptome and epigenome level.

2022-05-25 | GSE185004 | GEO

Induction of mouse totipotent stem cells by a defined chemical cocktail [bulk RNA-seq]

Project description:Induction of mouse totipotent stem cells by a defined chemical cocktail [bulk RNA-seq]

| PRJNA767355 | ENA

Induction of mouse totipotent stem cells by a defined chemical cocktail [scRNA-seq]

2022-05-25 | GSE185001 | GEO

Induction of mouse totipotent stem cells by a defined chemical cocktail [ATAC-seq]

2022-05-25 | GSE184999 | GEO

Induction of mouse totipotent stem cells by a defined chemical cocktail [bulk RNA-seq]

2022-05-25 | GSE185000 | GEO

Induction of mouse totipotent stem cells by a defined chemical cocktail

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-25 | GSE185005 | GEO

Global transcriptional analysis of mouse totipotent potential stem cells and extended pluripotent stem cells

Project description:It is challenging to derive totipotent stem cells in vitro that functionally and molecularly resemble cells from totipotent embryos. Here, we report that a chemical cocktail enables the derivation of totipotent-like stem cells, designated as totipotent potential stem (TPS) cells, from 2-cell mouse embryos and extended pluripotent stem cells that can be stably maintained long-term in vitro. TPS cells shared transcriptomic features with 2-cell mouse embryos and express totipotent markers. In vivo chimeric assays show that these cells have embryonic and extraembryonic developmental potentials at the single cell level. Moreover, we show that TPS cells can be induced into blastocyst-like structures resembling preimplantation mouse blastocysts. Our study demonstrates the feasibility of capturing and maintaining totipotency in vitro.

2022-05-04 | GSE183522 | GEO

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