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RNAseq analysis of adipose tissue transcriptomes from wild-type and FNIP1 adipocyte specific knockout (FNIP1-AKO) mice

ABSTRACT: RNAseq analysis of adipose tissue transcriptomes from wild-type and FNIP1 adipocyte specific knockout (FNIP1-AKO) mice

PROVIDER: PRJNA767577 | ENA |

REPOSITORIES: ENA

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RNAseq analysis of adipose tissue transcriptomes from wild-type and FNIP1 adipocyte specific knockout (FNIP1-AKO) mice

Project description:Activation of thermogenesis in brown and beige adipocytes upon activation by external stimuli burns fuel energy as heat. Owing to its remarkable benefits on metabolic health and its demonstrated presence in adult humans, beige adipocytes holds great promise to combat obesity and metabolic diseases. Folliculin interacting protein 1 (FNIP1) is an adaptor protein originally identified through its interaction with folliculin (FLCN) and AMPK. To investigate the physiological role of FNIP1 in adipose tissue in vivo, we generated adipocyte-specific FNIP1 deficient mice (referred to as FNIP1-AKO) by crossing mice bearing a conditional Fnip1 allele with introns 5 and 6 floxed (Fnip1 lox/lox) with the adiponectin promoter–driven Cre mice. We performed transcriptome analysis by whole-genome gene expression profiling experiments in inguinal white adipose tissue (iWAT) from both wild-type (WT) and FNIP1-AKO mice. We were particularly interested in the subset of genes that were up-regulated in the FNIP1-AKO mice. Gene ontology (GO) analysis revealed that the primary up-regulated genes were mitochondrial and lipid metabolism-related genes. Gene expression validation studies demonstrated that a broad array of genes involved in thermogenic and mitochondrial oxidative program was induced in FNIP1-AKO iWAT. Together, the transcriptional profiling results indicate that loss of FNIP1 activate thermogenic program in white adipocytes.

2022-02-26 | GSE185067 | GEO

Quantitative Analysis of Wild Type and Prmt1 adipocyte-specific KO Epididymal primary adipocyte Transcriptomes

Project description:We attempted to analyze the effect of adipocyte-specific knockout of Prmt1 in energy homeostasis in mice. By analyzing the transcriptomics of epididymal primary adipocytes from male mice with wild type and adipocyte-specific Prmt1 knockout mice, we provide the insight into the collective roles of Prmt1 in epididymal white adipose tissues.

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Quantitative Analysis of Wild Type and Crtc2 adipocyte-specific KO Epididymal primary adipocyte Transcriptomes

Project description:We attempted to analyze the effect of adipocyte-specific knockout of Crtc2 in in aging. By analyzing the transcriptomics of epididymal primary adipocytes from male mice with wild type and adipocyte-specific Crtc2 knockout mice in response to aging, we provide the insight into the collective roles of Crtc2 in epididymal white adipose tissues upon aging.

2022-07-09 | GSE207433 | GEO

RNAseq analysis of skeletal muscle transcriptomes from wild-type, FNIP1-knockout (FNIP1-KO) mice, and mice expressing FNIP1 only in skeletal muscle (FNIP1-TgKO).

Project description:This experiment was conducted to identify FNIP1-dependent mRNA transcripts alteration in skeletal muscle. Mouse FNIP1 was specifically overexpressed in skeletal muscle in FNIP1-transgenic (FNIP1-Tg) mice. FNIP1-Tg mice were crossed with FNIP1-knockout (FNIP1-KO) mice to generate FNIP1-TgKO mice expressing FNIP1 only in skeletal muscle but not in other tissues. The muscle glycolytic-to-oxidative transformation is determined, largely in part, at the level of gene expression. To gain insight into the FNIP1-dependent regulation of skeletal muscle energy metabolism, transcriptome analysis was performed by whole-genome gene expression profiling experiments in gastrocnemius (GC) muscle from both FNIP1-KO and FNIP1-TGKO mice. We were particularly interested in the subset of genes that were regulated only in the FNIP1-KO mice but not in FNIP1-TGKO mice. Gene ontology (GO) analysis revealed that the primary FNIP1-regulated genes were mitochondrial metabolic genes. A broad array of genes involved in mitochondria was regulated FNIP1-KO muscle in a FNIP1-dependent manner. Together, the transcriptional profiling results indicate that a significant subset of FNIP1 target genes, were those involved in mitochondrial function.

2021-03-17 | GSE150193 | GEO

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Project description:Transcriptiomics profiling of brown adipose tissue of adipocyte-specific Beclin1 knockout mice

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Analysis of gonadal fat from male control and adipocyte-specific LAMA4 knockout mice

Project description:To determine the transcriptomic effects of LAMA4 loss on adipose tissue structure and gene expression in two dietary paradigms, we assessed gonadal white adipose tissue of male control and adipocyte-specific LAMA4 knockout mice using a Quant-Seq strategy.

2022-08-21 | GSE179138 | GEO

Analysis of inguinal fat from male and female control and adipocyte-specific STAT5 knockout mice

Project description:To determine the transcriptomic effects of STAT5 loss on adipose tissue gene expression, we assessed inguinal white adipose tissue of 6-week old male and female control and adipocyte-specific STAT5 knockout mice using a Quant-Seq strategy.

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2020-05-26 | GSE148275 | GEO

RNAseq analysis of skeletal muscle transcriptomes from wild-type, FNIP1-knockout (FNIP1-KO) mice, and mice expressing FNIP1 only in skeletal muscle (FNIP1-TgKO).

Project description:RNAseq analysis of skeletal muscle transcriptomes from wild-type, FNIP1-knockout (FNIP1-KO) mice, and mice expressing FNIP1 only in skeletal muscle (FNIP1-TgKO).

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RNA-seq results in the adipose tissue of male adipocyte-specific histone H1.2 knockout mice

Project description:Our study represents the first detailed analysis of transcriptomes in iWAT and BAT of male adipocyte-specific H1.2 knockout mice to understand key pathways and regulatory genes, with biologic replicates, generated by RNA-seq technology

2023-06-15 | GSE215412 | GEO

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