Project description:Gene expression in A498 cells infected with shCtrl and shMCM8 was detected with a PrimeView human gene expression array. We performed Affymetrix Human Gene Chip Prime View analysis on shCtrl and shMCM8 A498 cells. Of the 2415 differentially expressed genes (DEGs) found in shMCM8 and shCtrl A498 cells, 1263 were up-regulated and 1152 were down-regulated based on the threshold of absolute fold change ≥ 2 and FDR < 0.05
Project description:Gene expression in HCT-116 cells infected with shCtrl and shRFWD3 was detected with a PrimeView human gene expression array. We performed Affymetrix Human Gene Chip Prime View analysis on shCtrl and shRFWD3 HCT 116 cells. Of the 1305 differentially expressed genes (DEGs) found in shRFWD3 and shCtrl HCT 116 cells, 629 were up-regulated and 676 were down-regulated based on the threshold of absolute fold change ≥ 2 and FDR < 0.05
Project description:In order to study the molecular mechanisms of malignant biological behavior in gastric cancer regulated by NCAPD3, GeneChip PrimeView human gene expression array was used to detect differentially expressed genes (DEGs) before and after NCAPD3 knockdown. We used microarrays to detail the effect of NCAPD3 knockdown on gene expression in gastric cancer cell and identified differentially expressed genes during this process.
Project description:miR-490 is robustly downregulated in GBM tumour samples. This study identifies the genes differentially expressed upon miR-490 overexpression in U87MG glioblastoma cell line. GeneChip PrimeView Human Gene Expression Array was used to assess mRNA expression profile in response to miR-490 overexpression in U87MG cell line.
Project description:Genome wide expression profiling of ulcerative colitis, and normal colon mucosa samples. The GeneChip PrimeView Human Gene Expression Array was used to obtain expresion profiles across colon mucosa samples. Samples came from 5 ulcerative colitis affected, and 5 normal individuals.
Project description:Comparison of the RNA expression profiles of CD14+ monocytes from human peripheral blood with derived dendritic cells (DCs) and macrophages (MACs) obtained by exposure with GM-CSF/IL-4 and GM-CSF, respectively, and with mature DCs and MACs after lipopolysaccharide (LPS) exposure The expression profiles of RNA of human CD14+ monocytes were compared with derived immature dendritic cells (iDCs) and macrophages (iMACs) following GM-CSF/IL-4 and GM-CSF incubation, and then activation/maturation with lypopolysaccharyde (LPS) using the Affymetrix PrimeView Human Gene Expression array (Affymetrix, Santa Clara, CA). This platform allows the interrogation of >36,000 transcrits and variants per sample. The samples were hybridized in the array following the manufacturer’s instructions.