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Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis

ABSTRACT: Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis

PROVIDER: PRJNA767836 | ENA |

REPOSITORIES: ENA

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Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis

Project description:Callogenesis, the process during which explants derived from differentiated plant tissues are subjected to a de-differentiation step characterized by the proliferation of an unorganized mass of cells, is fundamental to indirect organogenesis and the establishment of cell suspension cultures. There-fore, understanding how callogenesis takes place is helpful to plant tissue culture, as well as to plant biotechnology and bioprocess engineering. The common herbaceous plant stinging nettle (Urtica dioica L.) is a species producing cellulosic fibres (the bast fibres) and a whole array of phytochemicals of pharmacological and cosmeceutical use. It is thus of interest as a potential multi-purpose plant. In this study, callogenesis in internode explants of a nettle fibre clone (clone 13) was studied using RNA-Seq to understand which gene ontologies predominate at different time points. Callogenesis was induced with the plant growth regulators α-napthaleneacetic acid (NAA) and 6-benzyl aminopurine (BAP) after having determined their optimal concentrations. The process was studied over a period of 34 days, time point at which a well visible callus mass developed on the explants. The bioinformatic analysis of the transcriptomic dataset revealed specific gene ontologies charac-terizing each of the four time points investigated (0, 1, 10 and 34 days-D). The results show that while the advanced stage of callogenesis is characterized by the iron deficiency response triggered by the high levels of reactive oxygen species accumulated by the proliferating cell mass, the intermediate and early phases are dominated by ontologies related to immune response and cell wall loosening, respectively.

2021-11-15 | GSE185155 | GEO

Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis.

Project description: Not available

| S-EPMC8618292 | biostudies-literature

Transcriptomic analysis of pig intestinal explants exposed or not to deoxynivalenol

Project description:Intestinal explants from 5-weeks old piglets were exposed 4h to control or 10 µM deoxynivalenol-contaminated cell culture medium. Explants were nitrogen frozen. RNA were extracted, quantified and their quality was checked with Tapestation 4200 device. RNA samples were used to perform a microarray experiment

2021-06-30 | GSE165968 | GEO

Urticaceae

Project description:Various species of stinging nettle relatives Raw sequence reads

| PRJNA592832 | ENA

Transcriptomic analysis of pig intestinal explants exposed or not to deoxynivalenol

Project description:Transcriptomic analysis of pig intestinal explants exposed or not to deoxynivalenol

| PRJNA698790 | ENA

chick intermediate neural explants

Project description:chick intermediate neural explants

| PRJEB5921 | ENA

Transcriptional profiling by array of Amphiura filiformis arm explants during regeneration

Project description:Gene expression profiling of the proximal, medial and distal parts of Amphiura filiformis arm explants.

2012-01-11 | E-MEXP-3413 | biostudies-arrayexpress

Temporal transcriptomic changes during DSS colitis development and resolution.

Project description:Temporal transcriptomic changes during DSS colitis development and resolution.

| PRJEB41685 | ENA

Transcriptomic changes during regeneration in demosponge Cinachyyrella cf cavernosa

Project description:Transcriptomic changes during regeneration in demosponge Cinachyyrella cf cavernosa

| PRJNA556233 | ENA

Analysis of transcriptional differences after IFN-beta (IFN-b) or IFN-lambda (IFN-l) treated human mid-gestation chorionic villus explants

Project description:Purpose: Treatment of midgestation chorionic villus explants with recombinant IFN-b leads to morphological changes including formation of syncitial knots. However, treatment of explants with IFN-l, which is constitutively expressed by syncytiotrophoblasts, is not sufficient to cause these same changes. To understand the different transcriptional changes induced by IFN-b and IFN-l and underlying cause of these morphological changes, we performed RNAsequencing after these different treatments. Methods: Chorionic villi of 18-21 gestation week placentas were dissected. Explants were cultured in DMEM F/12 with 1000 U recombinant IFN-b or 1000U recombinant IFN-l for 24 hours. RNA was then isolated from explants, and RNAsequencing was performed to assess transcriptional changes. Results/ Conclusions: IFN-b but not IFN-l induces interferon stimulated genes (ISGs) in mid gestation chorionic villus explants.

2017-12-31 | GSE104348 | GEO

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