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Spatial transcriptome analysis of gastric cancer which GAN-KP transplanted C57BL/6J mouse

ABSTRACT: Spatial transcriptome analysis of gastric cancer which GAN-KP transplanted C57BL/6J mouse

PROVIDER: PRJNA773137 | ENA |

REPOSITORIES: ENA

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Spatial transcriptome analysis of gastric cancer which GAN-KP transplanted C57BL/6J mouse

Project description:We have now developed an organoid-based model of gastric cancer from GAstric Neoplasia (GAN) mice, which express Wnt1 and the enzymes COX2 and microsomal prostaglandin E synthase 1 in the stomach. Both p53 knockout (GAN-p53KO) organoids and KRASG12V-expressing GAN-p53KO (GAN-KP) organoids were generated by genetic manipulation of GAN mouse–derived tumor (GAN-WT) organoids. To uncover the molecular mechanism underlying the intratumoral heterogeneity of GAN-KP tumors, we performed spatial transcriptomics analysis with the 10× Genomics Visium platform, which allows characterization of the spatial topography of gene expression.

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Gene expression analysis of three tumor organoids (GAN-WT, GAN-p53 and GAN-KP) derived from gastric tumor in K19-Wnt1/C2mE (GAN) mouse.

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Expression data of mouse gastric tumors (TNF KO-Gan and BALB/c-Gan mice )

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Project description:Chaihu-Shugan-San (CSS) is a classical prescription used to treat depression. According to the traditional Chinese medicine (TCM) principle, the 2 decomposed recipes in CSS exert synergistic effects, including Shu Gan (stagnated Gan-Qi dispersion) and Rou Gan (pain alleviation). However, the specific mechanism and compatibility rule remain to be explored. We aimed to analyze the advantage of TCM compatibility in CSS prescriptions and explore its anti-depression mechanisms.

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