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Single-cell ATAC of WT thymic IEL precursors (IELp)

ABSTRACT: Single-cell ATAC of WT thymic IEL precursors (IELp)

PROVIDER: PRJNA773138 | ENA |

REPOSITORIES: ENA

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Gene expression in WT and LRF-deficient thymic IEL precusors (IELp) and their progeny CD8aa IEL

Project description:Gene expression in WT and LRF-deficient thymic IEL precusors (IELp) and their progeny CD8aa IEL

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Single-cell Gene expression of WT and LRF-deficient thymic IEL precusors (IELp)

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Single-cell ATAC of WT thymic IEL precursors (IELp)

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Gene expression in WT and LRF-deficient thymic IEL precusors (IELp) and their progeny CD8aa IEL

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Gene expression analysis of CD122HI versus CD122LO Vγ7+ murine small intestinal IEL at 14-17 days of age

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Gene expression analysis of CD122HI versus CD122LO VÎ³7+ murine small intestinal IEL at 14-17 days of age

Project description:Background: Recently have found that Btnl1 expressed by murine enterocytes shapes the local TCR-VÎ³7+ compartment by driving their clonotypic expansion and phenotypic maturation from CD122LO to CD122HI VÎ³7+ cells. The neonatal (D14-17) murine small intestinal epithelium is enriched for VÎ³7+ IEL displaying an â??immatureâ?? CD122LO cell surface phenotype. We believe these cells are precursors for CD122HI VÎ³7+ IEL. Method: To further characterise this putative product-progenitor relationship, CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from individual D14-D17 mice on four independent occasions and compared by total RNAseq. Conclusion: >3000 genes are differentially expressed between CD122HI and CD122LO murine Vg7+ neonatal IEL 4 independent Paired samples of CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from the small intestinal epithelium of pooled 14-17 day old mice. RNA was isolated from sorted samples. Gene expression analysis was performed by total RNAseq.

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ScRNAseq of human IEL and LPL T lymphocytes

Project description:We sequenced T cells from 2 control and 2 Crohn's disease patients We determined that in human Th17 T cells are highly enriched in the IEL compartment We identified discrete populations of CD8 and CD4 T cells, which are differently represented in IEL and LP

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