Project description:This experiment was used to determine the effect of a botanical insecticide upon gene expression profiles in Drosophila melanogaster. Adult female Drosophila (oregon-R strain) were treated with an ethylacetate extract of Piper nigrum (Piperaceae) seeds formulated in 99% ethanol. Treatment was topical, using a Potter's tower to administer a total of 2 mL of a 0.9mg/mL concentration. Control treatment was identical except flies were treated with 99% ethanol as a solvent control. Gene expression was studied four hours post-treatment. The design is a direct comparison between total RNA of treated and untreated insects. Sample size is two and two reverse label hybridizations were done. Each sample is a pool of 240 insects which received the same treatment simultaneously.
Project description:The purpose of this experiment was to assess the effect of a synergistic combination of natural pyrethrin and an ethylacetate extract of Piper nigrum seeds (a botanical insecticide). This effect was compared to the effects of P. nigrum or pyrethrin used alone. Due to the synergistic nature of the mixture, it was predicted that gene expression profiles in this treatment would reflect this effect. Keywords: insecticide response, stress-response Overall design: Three sets of comparisons were performed. Drosophila exposed to either P. nigrum, pyrethrin or pyrethrin plus P. nigrum were compared to Drosophila exposed to the carrier solvent ethanol. These three data sets were then compared.
Project description:In this study, we have evaluated the proteomic changes that occur in Piper nigrum L.(black pepper) after infection by the pathogen Phytophthora capsici. We report novel leaf proteins from black pepper identified by an integrated transcriptome-assisted label-free quantitative proteomics pipeline. Several previously described methods were used to create this data set. Detached leaves were inoculated with either mock treatment, or the oomycete pathogen and small tissue samples only around the site of inoculation were collected for protein sample preparations. In order to quantify protein abundance in the samples being compared, we used a label free method of spiking samples with a known ratio of pre-digested peptide samples to normalize endogenous protein abundance in the MS detection. Our study attempts to explain the basal immune components of black pepper when challenged with P. capsici.