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Next Generation Sequencing Facilitates Quantitative Analysis of LPS treated THP-1 cells' Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of LPS treated THP-1 cells' Transcriptomes

PROVIDER: PRJNA779446 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing Facilitates Quantitative Analysis of LPS treated THP-1 cells' Transcriptomes

Project description:In order to understand the consequences of the LPS from different bacterias, we analyzed RNA gene expression profiles in a human myeloid cell line THP-1 cells. RNA-seq transcriptome was analyzed in THP-1 cells treated with LPS from three bacteria B. fungorum, S. marcescens, and P. myrsinacearum.

2021-11-11 | GSE188578 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of dendritic cell Transcriptomes during LPS response

Project description:Purpose:The purpose of this study is to detect activated or silenced genes during LPS-induced dendritic cell. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse dendritic cells were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse GM-CSF and IL-4, immature DCs were obtained before or after LPS stimulation. Immature DCs were sorted respectively based on marker CD86 and Iab(MHCII) using flowcytrometer. DC mRNA profiles were generated by deep sequencing,using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation during LPS stimulation. DC mRNA profiles immature BMDCs were generated by deep sequencing

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Next Generation Sequencing Facilitates Quantitative Analysis of THP-1 cells under different stimulas.

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Next Generation Sequencing Facilitates Quantitative Analysis of dendritic cell Transcriptomes during LPS response

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Next Generation Sequencing Facilitates Quantitative Analysis of Manganese (II) or LPS treated Wild Type BMDC Transcriptomes

Project description:Purpose: The goals of this study are to compare the different functions of manganese (II) and LPS on the activation and maturation of antigen presenting cells-BMDCs. Methods: BMDCs were generated by culturing bone marrow cells with 20 ng/ml of IL-4 (Genscript) and 20 ng/ml of GM-CSF (Genscript) at 37°C and 5% CO2 for 7 days.Then BMDCs were treated with MnCl2 (200 μM) or LPS (100 ng/ml) for 20 h. Then mRNAs were purified, sequenced and analyzed. Results: Using an optimized data analysis workflow, we compared 20339 transcripts in BMDCs. Manganese (II) induced 1677 transcripts expression with a fold change ≥1.5 and LPS induced 1555 transcripts expression with a fold change ≥1.5. Conclusion: Manganese (II) activates the production of type I IFNs and the maturation of BMDCs. But there are also some differences between functions of manganese (II) and LPS on BMDCs.

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Next Generation Sequencing Facilitates Quantitative Analysis of METTL3 overexpression in THP-1 cells [m6A-Seq]

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Next Generation Sequencing Facilitates Quantitative Analysis of METTL3 overexpression in THP-1 cells [RNA-seq]

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Next-generation sequencing facilitates quantitative analysis of wild-type and Nrl(-/-) retinal transcriptomes.

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Expression and ChIP-seq analysis LPS stimulated THP-1 cells

Project description:This SuperSeries is composed of the following subset Series: GSE32141: Expression analysis LPS stimulated THP-1 cells in four paired samples GSE32324: ChIP-seq analysis LPS stimulated THP-1 cells Refer to individual Series

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Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

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