Project description:Mechanotransduction has been lately recognized as a major regulator of organ homeostasis under a myriad of pathological conditions. The role of Piezo1 in cardiac hypertrophy has never been explored. Here we performed RNA-Seq assay for wild type and Piezo1-Cko mice under sham or TAC surgery.
Project description:The most commonly used inbred mouse strain, C57BL/6J, lacks a functional nicotinamide nucleotide transhydrogenase (Nnt) and thus, is protected from pressure-overload-induced oxidative stress and heart failure. We screened for differential gene expression in left ventricular myocardium after sham/transverse aortic constriction (TAC) surgery in 10-12 weeks old mice from BL/6N (functional Nnt) and BL/6J (non-functional Nnt, missense of exons 7-11) strains.
Project description:A number of mediators that have been found to be involved in the pathogenesis of cardiac hypertrophy affecting gene transcription, protein synthesis, metabolism,autophagy, oxidative stress and inflammation. These mediators including nuclear transcription factor, microRNAs and long noncoding RNAs. However, the gene expression profiles signaling pathways in the pressure overload-induced cardiac hypertrophy remain unknown. All mice were randomly undertaken sham surgery or TAC for 2 weeks. Hearts were harvested 2 weeks following TAC and performed to further analysis.
Project description:The long-non coding RNA NRON is enriched in cardiomyocytes where it localizes in the nucleus. Unexpectedly, NRON-overexpression in a transverse aortic constriction-induced hypertrophy mouse model exacerbated hypertrophy, whereas cardiomyocyte-specific NRON-deletion attenuated cardiac hypertrophy, thereby suggesting a novel regulatory role of NRON in the heart.
Project description:4 Adult male Sprague-Dawley rats (275-350 g) were anesthetized and subjected to hepatectomy sham surgery (abdominal cavity was opened, liver was handled, but no tissue resection was made). 1 hour after the surgery rats were killed and liver samples were harvested. This study was conducted to analyzes the effects of surgical stress on gene expression levels in rat liver. It provides additional data to 1-6 h partial hepatectomy study (Series GSE7415). Keywords: 1h hepatectomy sham surgery
Project description:miRNA pattern of cardic myocytes, cardiac fibroblasts and cardiac endothelial cells isolated from adult mice hearts at different time points after transaortic constriction (TAC) or sham surgery.
Project description:In this study we sought to characterize the long tern response to RYGB surgery using diet induced obese mice. Controls were sham operated mice. Mice were age, sex, weight and diet matched.
Project description:In this study we sought to characterize the acute response to RYGB surgery using diet induced obese mice. Controls were sham operated mice. Mice were age, sex, weight and diet matched.